1. 重组蛋白
  2. Receptor Proteins
  3. GPVI 蛋白, Mouse (HEK293, His)

GPVI 蛋白, Mouse (HEK293, His)

目录号: HY-P70180
COA 产品使用指南

GPVI 蛋白是一种血小板膜蛋白,分子量约为 62 kDa,是一种生理胶原蛋白受体。GPVI 以与同型二聚体 Fc 受体 y 链的复合物形式存在。GPVI 必须形成这样的二聚体复合物才能表现出与胶原蛋白的高亲和力结合。GPVI诱导的激活机制是由FcRγ链的免疫受体酪氨酸激活基序 (ITAM) 的酪氨酸磷酸化启动的,然后该信号主要通过酪氨酸磷酸化转导到许多相关蛋白质。血小板与胶原蛋白通过受体 GPVI 相互作用,导致血小板活化和粘附。GPVI 蛋白, Mouse (HEK293, His) 是重组的 GPVI 蛋白,由 HEK293 表达,带有 C-6*His 标签。GPVI 蛋白, Mouse (HEK293, His) 全长 244 个氨基酸,分子量为 40-60 kDa。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥4000 In-stock
100 μg   询价  

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描述

GPVI Protein is a platelet membrane protein with a molecular weight of ~62 kDa that is a physiological collagen receptor. GPVI is present as a complex with the homodimeric Fc receptor y-chain. GPVI must form such a dimeric complex to exhibit high affinity binding to collagen. The GPVI-induced activation mechanism is initiated by tyrosine phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of the FcR gamma-chain, and then this signal is transduced to many related proteins, mainly by tyrosine phosphorylation. Interaction of platelets with collagen via the receptor GPVI results in platelet activation and adhesion[1][2][3]. GPVI Protein, Mouse (HEK293, His) is the recombinant mouse-derived GPVI protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GPVI Protein, Mouse (HEK293, His) is 244 a.a., with molecular weight of 40-60 kDa.

研究背景

血小板与胶原蛋白通过受体 GPVI 相互作用,导致血小板活化和粘附,这是血栓形成所必需的过程。在血小板表面,GPVI 以与同型二聚体 Fc 受体 y 链的复合物形式存在(FcRgamma 的化学计量可能由两个 GPVI 分子和一个 FcRgamma 二聚体组成)。当胶原蛋白与 GPVI 结合时,FcRgamma 免疫受体酪氨酸激活基序的酪氨酸磷酸化会引发血小板激活级联,这种磷酸化诱导由许多信号转导蛋白组成的大型复合物的形成。GPVI抑制剂能够抑制血栓形成,但仍不会引起明显的出血倾向[1]
胶原蛋白和GPVI都是不溶性分子。GPVI的胞外结构域是如下可溶形式:单体形式(GPVIex)和与人免疫球蛋白Fc结构域融合的GPVI二聚体形式(GPVI-Fc2)。纯化的 GPVIex 强烈抑制惊厥素 (Cvx) 诱导的血小板聚集,但仅微弱地抑制胶原相关肽诱导的血小板聚集。然而,只有 GPVI-Fc2 能够抑制胶原蛋白诱导的血小板聚集,而 GPVIex 则不能[3]

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

B2RR15 (Q22-K265)

基因 ID

243816

蛋白结构
N-term
GPVI (Q22-K265)
Accession # B2RR15
6*His
C-term
同用名
rMuGlycoprotein 6 (Platelet)/GPVI, His ; Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI
氨基酸序列

QSGPLPKPSLQAQPSSLVPLGQSVILRCQGPPDVDLYRLEKLKPEKYEDQDFLFIPTMERSNAGRYRCSYQNGSHWSLPSDQLELIATGVYAKPSLSAHPSSAVPQGRDVTLKCQSPYSFDEFVLYKEGDTGPYKRPEKWYRANFPIITVTAAHSGTYRCYSFSSSSPYLWSAPSDPLVLVVTGLSATPSQVPTEESFPVTESSRRPSILPTNKISTTEKPMNITASPEGLSPPIGFAHQHYAK

分子量

40-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GPVI Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GPVI Protein, Mouse (HEK293, His)
目录号:
HY-P70180
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