2. 重组蛋白
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA1/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His)

HA1/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His)

目录号: HY-P74033
产品使用指南

HA/血凝素蛋白结合细胞表面的受体，促进病毒附着。它通过内吞作用导致内化。HA 决定宿主范围和毒力，介导病毒和内体膜的融合。HA1/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His) 是重组的 HA/血凝素蛋白，由 HEK293 表达，带有 C-His 标签。

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50 μg   询价  
100 μg   询价  

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描述

The HA/Hemagglutinin protein binds receptors on the cell surface, facilitating virus attachment.It leads to internalization via endocytosis.HA determines host range and virulence, mediating fusion of virus and endosomal membrane.HA2 undergoes conformational change in endosomes, releasing fusion peptide and forming fusion pore.Multiple HA trimers are required for pore formation.HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.
研究背景

HA/血凝素蛋白与细胞表面上含唾液酸的受体结合，促进病毒颗粒附着到细胞上。这种附着导致大约三分之二的病毒颗粒通过网格蛋白依赖性内吞作用内化，而其余三分之一遵循网格蛋白和小窝蛋白独立途径。该蛋白质在确定宿主范围限制和毒力方面起着至关重要的作用。作为I类病毒融合蛋白，它通过介导内吞病毒颗粒膜与内体膜的融合，负责病毒渗透到细胞质中。在内体的低pH环境下，HA2发生不可逆的构象变化，导致融合疏水肽的释放并形成有能力的融合孔。HA 的多个三聚体对于该孔的形成是必需的。
种属

Virus
表达系统

HEK293
标签

C-His
蛋白编号

P03453 (D18-R344)
基因 ID

/
蛋白结构
N-term
HA1/Hemagglutinin (D18-R344)
Accession # P03453
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/USSR/90/1977, HEK293, His)
分子量

Approximately 38.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HA1/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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