HCC-4/CCL16 蛋白, Human

HCC-4/CCL16 Protein, Human is a CC chemokine that specifically attracts lymphocytes, dendritic cells and monocytes, increases their adhesion and has myelosuppressive activity. HCC-4/CCL16 Protein, Human interacts with CCR1, CCR2, CCR5 and CCR8 to mediate inflammatory and cancer responses. HCC-4/CCL16 Protein, Human is a recombinant human HCC-4/CCL16 (Q24-Q120) expressed by E. coli^[1].

CCL16，也称为 CC 趋化因子 4、肝表达趋化因子 (LEC) 和 monotactin-1 (MTN-1)，是属于 CC 趋化因子家族（位于人类 17 号染色体上的趋化因子簇）的一种小细胞因子。组胺 H4 受体的配体。CCL16 可以趋化单核细胞和淋巴细胞，但不能趋化中性粒细胞。最近的研究表明，CCL16 增加肿瘤排斥、巨噬细胞的抗原呈递以及血管内皮细胞的血管生成活性。另一方面，CCL16 还可能增强细胞毒性 T 细胞和树突状细胞 (DC) 淋巴细胞的抗癌作用^[1][2]。

CCL16 expression is upregulated in LPS-induced WI-38 cells, and CCL16 silencing inhibits LPS-induced apoptosis and inflammation in human lung fibroblast line WI-38 cells^[2].
HCC-4/CCL16 (1-3000 ng/mL, 12 h) induces maximal chemotactic activity in CCR1-expressing HOS cells at a concentration of 1000ng/mL, transducing chemotactic signals via G i / Go proteins, PLC and PKCδ. It also activates the p38 MAPK signaling pathway in a time- and dose-dependent manner but with no effect on Ca²⁺ mobilization^[3].

1. Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/mL.
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 1.25 μg/mL, corresponding to a specific activity is 800 U/mg.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 1.25 μg/mL, corresponding to a specific activity is 800 U/mg.

Human

E. coli

Tag Free

O15467 (Q24-Q120)

6360  [NCBI]

QPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNPNDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ

10-15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jan Korbecki, et al. CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of the Ligands of Receptors CCR1, CCR2, CCR3, and CCR4. Int J Mol Sci. 2020 Nov 9;21(21):8412.  [Content Brief]

  [2]. Strasly M, et al. CCL16 activates an angiogenic program in vascular endothelial cells. Blood. 2004 Jan 1;103(1):40-9.  [Content Brief]

  [3]. Jiahui Xu, et al. Silencing XIST mitigated lipopolysaccharide (LPS)-induced inflammatory injury in human lung fibroblast WI-38 cells through modulating miR-30b-5p/CCL16 axis and TLR4/NF-κB signaling pathway. Open Life Sci. 2021 Feb 6;16(1):108-127.  [Content Brief]

  [4]. In Sik Kim, et al. Differential CCR1-mediated chemotaxis signaling induced by human CC chemokine HCC-4/CCL16 in HOS cells. FEBS Lett. 2005 Nov 7;579(27):6044-8.  [Content Brief]