HCK 蛋白, Human (Sf9, His)

The HCK protein is a hematopoietic cell-dominated non-receptor tyrosine protein kinase that is critical for innate immune responses in neutrophils, monocytes, macrophages, and mast cells. HCK Protein, Human (Sf9, His) is the recombinant human-derived HCK protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of HCK Protein, Human (Sf9, His) is 446 a.a., .

HCK 蛋白是一种主要存在于造血细胞中的非受体酪氨酸蛋白激酶，作为传递来自各种细胞表面受体的信号的关键介质，在调节先天免疫反应中发挥着至关重要的作用。它复杂地调节中性粒细胞、单核细胞、巨噬细胞和肥大细胞的功能，影响吞噬作用、细胞存活、增殖、粘附和迁移等过程。HCK 作用于 FCGR1A、FCGR2A、CSF3R、PLAUR、IFNG、IL2、IL6、IL8 以及整合素 ITGB1 和 ITGB2 等受体的下游，在吞噬过程中协调关键事件，包括分泌溶酶体的动员、脱粒和 NADPH 氧化酶的激活，导致呼吸爆发和炎症分子的释放。此外，HCK 还促进肌动蛋白细胞骨架的重组、肌动蛋白聚合、足小体的形成和细胞突起。它抑制 TP73 介导的转录激活和细胞凋亡，同时磷酸化关键信号蛋白，如 CBL、ADAM15、BCR、ELMO1、FCGR2A、GAB1、GAB2、RAPGEF1、STAT5B、TP73、VAV1 和 WAS，突显其广泛参与多种细胞途径。

Human

Sf9 insect cells

N-8*His

P08631 (I81-P526, Q523E, Q524E, Q525I)

3055

Approximately 53.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.