1. 重组蛋白
  2. Viral Proteins
  3. HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His)

HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His)

目录号: HY-P76388
产品使用指南

HCoV-HKU1 Spike/S1 蛋白通过将病毒颗粒附着在宿主细胞膜上并启动感染过程,在感染中发挥关键作用。作为 I 类病毒融合蛋白,它通过动态构象转变促进病毒粒子和宿主细胞之间的膜融合。HCoV-HKU1 Spike/S1 蛋白 (Q5MQD0, HEK293, His) 是重组的 HCoV-HKU1 刺突/S1 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The HCoV-HKU1 Spike/S1 protein plays a key role in infection by attaching viral particles to the host cell membrane, initiating the infection process. As a class I viral fusion protein, it promotes membrane fusion between virions and host cells through dynamic conformational transitions. HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) is the recombinant Virus-derived HCoV-HKU1 Spike/S1 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

HCoV-HKU1 Spike/S1 蛋白通过与宿主受体相互作用将病毒体附着到宿主细胞膜上,从而启动感染,从而在感染过程中发挥关键作用。作为 I 类病毒融合蛋白,它促进病毒粒子和细胞膜的融合。该蛋白质经历动态构象转变,表现出至少三种状态:融合前天然状态、发夹前中间状态和融合后发夹状态。在融合过程中,以七肽重复为特征的卷曲线圈区域形成发夹三聚体结构。这种排列将融合肽定位在紧邻胞外域的C端区域的位置,这种结构被认为能够驱动病毒和靶细胞膜的并置和随后的融合。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q5MQD0/YP_173238.1 (G16-R760)

基因 ID

3200426  [NCBI]

蛋白结构
N-term
HCoV-HKU1 S1 (G16-R760)
Accession # Q5MQD0/YP_173238.1
His
C-term
同用名
Human coronAvirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein (S1 Subunit, His)
分子量

130-140 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HCoV-HKU1 Spike/S1 Protein (Q5MQD0, HEK293, His)
目录号:
HY-P76388
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