2. 重组蛋白
  3. Viral Proteins
  4. HCoV-NL63 Spike/S 蛋白 (APF29071, sf9, His)

HCoV-NL63 Spike/S 蛋白 (APF29071, sf9, His)

目录号: HY-P77383
产品使用指南

HCoV-NL63 是一种来自冠状病毒 A 属的丝状病毒。HCoV-NL63 是一种有包被的阳性单链 RNA 病毒，通过与 ACE2 结合进入宿主细胞。HCoV-NL63 主要感染幼儿、老年人和免疫功能低下的急性呼吸道疾病患者。HCoV-NL63 Spike/S 蛋白 (APF29071, sf9, His) 是重组的 HCoV-NL63 刺突/S 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。HCoV-NL63 Spike/S 蛋白 (APF29071, sf9, His) 全长 1296 个氨基酸，分子量约为 ~142.4 KDa。

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描述

HCoV-NL63 is a coronavirus, specifically a filovirus from the genus coronavirus A. HCoV-NL63 is a coated, positive single-stranded RNA virus that enters host cells by binding to ACE2. HCoV-NL63 is mainly found in young children, the elderly, and immunocompromised patients with acute respiratory diseases. HCoV-NL63 Spike/S Protein (APF29071, sf9, His) is the recombinant Virus-derived HCoV-NL63 Spike/S protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HCoV-NL63 Spike/S Protein (APF29071, sf9, His) is 1296 a.a., with molecular weight of ~142.4 KDa.
研究背景

HCoV-NL63 是一种冠状病毒，特别是一种来自甲型冠状病毒属的丝状病毒。该病毒是一种包膜、阳性意义的单链 RNA 病毒，通过与 ACE2 结合进入宿主细胞。S1 区通过与宿主受体的相互作用将病毒粒子附着在细胞膜上，从而引发感染。与受体结合可能引起 S 糖蛋白的构象变化，揭示 S2 区融合肽，激活膜融合。S2 区属于 I 类病毒融合蛋白。该病毒主要见于幼儿、老年人和患有急性呼吸道疾病的免疫功能低下患者。
种属

Virus
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

APF29071 (M1-P1296)
基因 ID

/
蛋白结构
N-term
HCoV-NL63 S (M1-P1296)
Accession # APF29071
His
C-term
同用名
Human coronAvirus (HCoV-NL63) Spike Protein (S1+S2, His)
分子量

Approximately 142.4 kDa.
纯度

Greater than 80% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HCoV-NL63 Spike/S Protein (APF29071, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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