HDAC6 蛋白, Human (His)

The HDAC6 protein coordinates multiple functions, deacetylating histones and proteins such as tubulin and CTTN. Its role in epigenetic repression affects transcription, cell cycle, and development. HDAC6 Protein, Human (His) is the recombinant human-derived HDAC6 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HDAC6 Protein, Human (His) is 352 a.a., with molecular weight of ~42.4 kDa.

HDAC6 蛋白承担多方面的作用，主要负责核心组蛋白（H2A、H2B、H3 和 H4）N 端部分赖氨酸残基的脱乙酰化，有助于表观遗传抑制，在转录调控、细胞周期进展和发育事件。组蛋白脱乙酰酶（包括 HDAC6）在大型多蛋白复合物中运作，协调这些过程。除了组蛋白之外，HDAC6 还将其去乙酰化活性扩展到多种蛋白质，例如微管蛋白（对于微管依赖性细胞运动至关重要）和 α-微管蛋白（对于纤毛分解至关重要）。此外，HDAC6 通过 CTTN 去乙酰化促进肌动蛋白聚合和自噬体-溶酶体融合，促进自噬完成。它参与 MTA1 介导的乳腺癌 ESR1 表达的表观遗传调控和促进成牙本质细胞分化，展示了其多种功能。此外，HDAC6在错误折叠蛋白的降解中发挥着关键作用，当分子伴侣重折叠系统和泛素蛋白酶体不足时，HDAC6充当识别多泛素化错误折叠蛋白的接头，并将其靶向聚集体，通过自噬清除。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9UBN7-1 (M489-E840)

10013  [NCBI]

MMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVE

Approximately 42.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.