1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H3N2 (P03437, sf9, His)

HA/Hemagglutinin 蛋白, H3N2 (P03437, sf9, His)

目录号: HY-P75079
产品使用指南

HA/血凝素蛋白与细胞表面受体结合,促进病毒附着。它决定宿主范围和毒力。作为 I 类融合蛋白,它介导病毒和内体膜的融合以进行细胞渗透。酸性内体触发 HA2 构象变化,释放融合肽。融合孔的形成需要多个HA三聚体。HA/Hemagglutinin 蛋白, H3N2 (P03437, sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

HA/Hemagglutinin Protein binds to cell surface receptors, facilitating virus attachment.It determines host range and virulence.As a class I fusion protein, it mediates fusion of virus and endosomal membranes for cell penetration.Acidic endosomes trigger HA2 conformational change, releasing fusion peptide.Multiple HA trimers are needed for fusion pore formation.HA/Hemagglutinin Protein, H3N2 (P03437, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

HA/血凝素蛋白与细胞表面上含唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着导致大约三分之二的病毒颗粒通过网格蛋白依赖性内吞作用内化,大约三分之一通过网格蛋白和小窝蛋白独立途径内化。它在确定宿主范围限制和毒力方面起着至关重要的作用。作为I类病毒融合蛋白,它负责介导内吞的病毒颗粒膜与内体膜的融合,使病毒能够渗透到细胞的细胞质中。内体中的酸性环境触发HA2的不可逆构象变化,导致融合疏水肽的释放。HA/血凝素蛋白的多个三聚体对于形成功能性融合孔是必需的。

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P03437 (Q17-W530)

基因 ID

/

蛋白结构
N-term
HA/Hemagglutinin (Q17-W530)
Accession # P03437
His
C-term
同用名
Influenza A H3N2 (A/Aichi/2/1968) Hemagglutinin / HA Protein (sf9, His)
分子量

Approximately 59 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H3N2 (P03437, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H3N2 (P03437, sf9, His)
目录号:
HY-P75079
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