1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H5N1 (G2U0T8, HEK293, His)

HA/Hemagglutinin 蛋白, H5N1 (G2U0T8, HEK293, His)

目录号: HY-P75032
产品使用指南

HA/血凝素蛋白与唾液酸受体结合,启动病毒附着于细胞。内化通过网格蛋白依赖性或不依赖性途径发生。HA/Hemagglutinin 蛋白, H5N1 (G2U0T8, HEK293, His) 是重组的 HA/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The HA/hemagglutinin protein binds to sialic acid receptors, initiating viral attachment to cells.Internalization occurs via clathrin-dependent or -independent pathways.HA/Hemagglutinin Protein, H5N1 (G2U0T8, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

血凝素蛋白 (HA) 与细胞表面上含唾液酸的受体结合,启动病毒颗粒附着到细胞上。这种附着导致病毒粒子的内化,通过网格蛋白依赖性内吞作用或通过网格蛋白和小窝蛋白独立途径。HA 在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。作为I类病毒融合蛋白,HA负责通过介导内吞病毒颗粒膜与内体膜的融合来促进病毒渗透到细胞质中。在内体的低pH环境下,HA2发生不可逆的构象变化,导致融合疏水肽的释放。需要多个HA三聚体才能形成有能力的融合孔。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

G2U0T8 (D17-Q530)

基因 ID

/

蛋白结构
N-term
HA/Hemagglutinin (D17-Q530)
Accession # G2U0T8
His
C-term
同用名
Influenza A H5N1 (A/Hubei/1/2010) Hemagglutinin / HA Protein (HEK293, His)
分子量

65-70 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H5N1 (G2U0T8, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H5N1 (G2U0T8, HEK293, His)
目录号:
HY-P75032
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