1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H7N3 (CAF33017, sf9, His)

HA/Hemagglutinin 蛋白, H7N3 (CAF33017, sf9, His)

目录号: HY-P75883
产品使用指南

禽流感病毒 (AIV) 属于正粘病毒属,含有八个单链负义RNA片段 (PB2、PB1、PA、HA、NP、NA、M和NS)。血凝素 (HA) 蛋白呈现为同源三聚体,其中每个单体在 HA0 前体裂解后由两个二硫键连接的 HA1 和 HA2 亚基组成。HA蛋白负责受体结合和膜融合,在流感病毒进入途径中起着关键作用。HA/Hemagglutinin 蛋白, H7N3 (CAF33017, sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

Avian influenza virus (AIV) belongs to the genus Orthomyxovirus and contains eight single-stranded negative-sense RNA segments (PB2, PB1, PA, HA, NP, NA, M, and NS). The haemagglutinin (HA) protein presents as a homotrimer in which each monomer consists of two di-sulfide-linked HA1 and HA2 subunits after the cleavage of the HA0 precursor. The HA protein, which is responsible for receptor binding and membrane fusion, plays a key role in the influenza virus entry pathway[1][2]. HA/Hemagglutinin Protein, H7N3 (CAF33017, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

禽流感 (AI) 病毒属于正粘病毒科、甲型流感病毒属,并含有负义八段 RNA 基因组(聚合酶基本 2,PB2;聚合酶基本 1,PB1;聚合酶酸,PA;血凝素,HA;核衣壳蛋白,NP,神经氨酸酶,NA;矩阵,M;非结构蛋白,NS)。LPAI病毒 H5 和 H7 亚型的 HA 裂解位点中碱性氨基酸的多次替换或插入使其能够被常见的细胞蛋白酶裂解,并且突变的病毒可以在宿主中全身传播,导致高致病性表型[1]
一些研究已证实,去除 HA 的 N-连接糖基化序列有助于增加 HA 与 α-2,6-连接唾液酸受体的亲和力,并减少病毒的生长和传播。不同 AIV 毒株的病毒脱落和传播差异取决于特定的 HA 和/或 NA 蛋白[2]

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

CAF33017 (D19-V524)

基因 ID

/

蛋白结构
N-term
HA/Hemagglutinin (D19-V524)
Accession # CAF33017
His
C-term
同用名
Influenza A H7N3 (A/turkey/Italy/214845/2002) Hemagglutinin / HA Protein (His)
分子量

Approximately 59.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H7N3 (CAF33017, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HA/Hemagglutinin Protein, H7N3 (CAF33017, sf9, His)
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