1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H9N2 (Q9ICY5, HEK293, His)

HA/Hemagglutinin 蛋白, H9N2 (Q9ICY5, HEK293, His)

目录号: HY-P74448
产品使用指南

HA/血凝素蛋白与唾液酸受体结合并促进病毒附着。它通过网格蛋白依赖性或独立途径触发内化。HA/Hemagglutinin 蛋白, H9N2 (Q9ICY5, HEK293, His) 是重组的 HA/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

HA/hemagglutinin protein binds to sialic acid receptors and promotes viral attachment. It triggers internalization through clathrin-dependent or independent pathways. HA/Hemagglutinin Protein, H9N2 (Q9ICY5, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag.

研究背景

HA蛋白与细胞表面含有唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着通过网格蛋白依赖性内吞作用或通过网格蛋白和小窝蛋白独立途径触发病毒的内化。HA蛋白对于确定病毒的宿主范围限制和毒力至关重要。作为I类病毒融合蛋白,它负责介导内吞病毒颗粒膜与内体膜的融合,使病毒渗透到细胞质中。在内体的低pH环境下,HA2发生不可逆的构象变化,导致融合疏水肽的释放。需要多个HA三聚体才能形成有能力的融合孔。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

Q9ICY5/NP_859037.1 (D19-K523)

基因 ID

1460996  [NCBI]

蛋白结构
N-term
H9N2 HA (D19-K523)
Accession # Q9ICY5/NP_859037.1
His
C-term
同用名
Influenza A H9N2 (A/Hong Kong/1073/99) Hemagglutinin / HA Protein (His)
分子量

65-75 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA/Hemagglutinin Protein, H9N2 (Q9ICY5, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H9N2 (Q9ICY5, HEK293, His)
目录号:
HY-P74448
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