1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA1/Hemagglutinin 蛋白, H10N3 (AGO87051, HEK293, His)

HA1/Hemagglutinin 蛋白, H10N3 (AGO87051, HEK293, His)

目录号: HY-P75870
产品使用指南

甲型流感病毒是正粘病毒科的单链、负义、分段RNA病毒,它的基因组包含 8 个病毒 RNA (vRNA) 片段,编码至少 17 种蛋白质。HA 作为受体结合糖蛋白发挥作用,与细胞表面唾液酸化糖蛋白结合。HA1/Hemagglutinin 蛋白, H10N3 (AGO87051, HEK293, His) 是重组的 HA1/血凝素蛋白,由 HEK293 表达,带有 C-His 标签。HA1/Hemagglutinin 蛋白, H10N3 (AGO87051, HEK293, His) 全长 340 个氨基酸,分子量约为 ~36.8 kDa。

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描述

Influenza A virus is a single-stranded, negative-sense, segmented RNA virus of the family Orthomyxoviridae. Its genome harbours eight viral RNA (vRNA) segments encoding at least 17 proteins. HA functions as the receptor binding glycoprotein that binds to cell-surface sialylated glycoproteins[1][2][3]. HA1/Hemagglutinin Protein, H10N3 (AGO87051, HEK293, His) is the recombinant Virus-derived HA1/Hemagglutinin protein, expressed by HEK293 , with C-His labeled tag. The total length of HA1/Hemagglutinin Protein, H10N3 (AGO87051, HEK293, His) is 340 a.a., with molecular weight of ~36.8 kDa.

研究背景

根据其两种表面糖蛋白血凝素 (HA) 和神经氨酸酶 (NA) 的不同抗原性,甲型流感病毒分为18种不同的HA亚型和11种不同的NA亚型。流感病毒在复制过程中容易发生基因突变,有时一两个氨基酸突变就能引起巨大的生物学变化[1]
HA 作为受体结合糖蛋白发挥作用,与细胞表面唾液酸化糖蛋白结合。特别地,流感病毒主要与大量位于鸟类下呼吸道和肠道中的α2,3-连接的唾液酸 (SA) 受体结合,对于禽流感病毒而言,与α2,6-连接的SA受体结合。病毒附着后,病毒和内体膜之间的融合反应需要HA蛋白发生构象变化,之后病毒核糖核蛋白被释放到受感染细胞的细胞质中[2]

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

AGO87051 (M1-R340)

基因 ID

/

蛋白结构
N-term
H10N3 HA1 (M1-R340)
Accession # AGO87051
His
C-term
同用名
Influenza A H10N3 (A/duck/Hunan/S11205/2012) Hemagglutinin Protein (HA1 Subunit) (His)
分子量

Approximately 36.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HA1/Hemagglutinin Protein, H10N3 (AGO87051, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HA1/Hemagglutinin Protein, H10N3 (AGO87051, HEK293, His)
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