Heparan Sulfate-6-O-Sulfotransferase-3 蛋白, Mouse (HEK293, His)

Heparan Sulfate-6-O-Sulfotransferase-3 (HS6ST3) is an enzyme involved in modifying heparan sulfate proteoglycan biosynthesis. It is predicted to be a membrane-bound integral component. HS6ST3 shows specific expression in the adult cortex (RPKM 1.3) and frontal lobe (RPKM 0.8), implying its potential role in determining the sulfation patterns of heparan sulfate in these contexts. Heparan Sulfate-6-O-Sulfotransferase-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Heparan Sulfate-6-O-Sulfotransferase-3 protein, expressed by HEK293 , with N-His labeled tag.

Heparan Sulfate-6-O-Sulfotransferase-3 (HS6ST3) 是一种具有硫酸乙酰肝素 6-O-磺基转移酶活性的蛋白质，参与硫酸乙酰肝素蛋白聚糖生物合成过程的酶促修饰。HS6ST3 预计作为膜的一个组成部分位于膜中，在各种组织中表现出偏向表达，包括成体皮层 (RPKM 1.3) 和成体额叶 (RPKM 0.8)，这表明它在塑造体内硫酸乙酰肝素的硫酸化模式中具有潜在作用。这些背景。

Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is 73.892 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Mouse

HEK293

N-6*His

Q9QYK4/NP_056635 (P28-W470)

50787  [NCBI]

PSCTSSCTNFGEQLRSGEARPPAVPSPARRAQAPLDEWERRPQLPPPPRGPPEGSRGVAAPEDEDEDPGDPEEEEEEEEEEPDPEAPENGSLPRFVPRFNFTLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCSYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVDINDGARQHIEELNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRAHRWPKEDRAMEGTVTEDYNSQVVRW

Approximately 75-110 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.