Heparinase III 蛋白, P. heparinus (His)

Heparinase III Protein is the unique member of the heparinase family of heparin-degrading lyases that recognizes the ubiquitous cell-surface heparan sulfate proteoglycans as its primary substrate. Heparinase III functions in cleaving metazoan heparan sulfate and providing carbon, nitrogen and sulfate sources for microorganisms. Furthermore, Heparinase III is cardioprotective in a dose-dependent manner, preserves endothelial function and attenuates PMN adherence to the coronary vascular endothelium. Heparinase III Protein, P. heparinus (His) is the recombinant Heparinase III protein, expressed by E. coli , with N-6*His labeled tag. The total length of Heparinase III Protein, P. heparinus (His) is 635 a.a., with molecular weight of ~74 kDa.

肝素酶 III 是肝素降解裂解酶肝素酶家族的独特成员，它识别普遍存在的细胞表面硫酸乙酰肝素蛋白聚糖作为其主要底物。肝素酶III的功能是裂解后生动物的硫酸乙酰肝素，并为微生物提供碳、氮和硫酸盐源。肝素酶 III 特异性裂解酸性多糖中富含硫酸乙酰肝素的区域。它主要作用于 N-硫酸化或 N-乙酰化葡萄糖胺（GlcNS 或 GlcNAc）与葡萄糖醛酸（GlcA）之间的糖苷键，作为主要位点，前提是 C2 GlcA 未硫酸化。它可以通过影响动脉损伤后硫酸乙酰肝素蛋白聚糖结合生长因子来限制增殖。此外，肝素酶 III 具有剂量依赖性的心脏保护作用，可保护内皮功能并减弱 PMN 对冠状血管内皮的粘附^[1][2][3][4]。

Measured by its ability to liberate oligosaccharides from heparin. Which can be measured by absorbance at 232 nm. The specific activity is 432.279935 pmol/min/µg, as measured under the described conditions.

Others

E. coli

N-6*His

AAB18278 (Q25-P659)

8254931

QSSSITRKDFDHINLEYSGLEKVNKAVAAGNYDDAAKALLAYYREKSKAREPDFSNAEKPADIRQPIDKVTREMADKALVHQFQPHKGYGYFDYGKDINWQMWPVKDNEVRWQLHRVKWWQAMALVYHATGDEKYAREWVYQYSDWARKNPLGLSQDNDKFVWRPLEVSDRVQSLPPTFSLFVNSPAFTPAFLMEFLNSYHQQADYLSTHYAEQGNHRLFEAQRNLFAGVSFPEFKDSPRWRQTGISVLNTEIKKQVYADGMQFELSPIYHVAAIDIFLKAYGSAKRVNLEKEFPQSYVQTVENMIMALISISLPDYNTPMFGDSWITDKNFRMAQFASWARVFPANQAIKYFATDGKQGKAPNFLSKALSNAGFYTFRSGWDKNATVMVLKASPPGEFHAQPDNGTFELFIKGRNFTPDAGVFVYSGDEAIMKLRNWYRQTRIHSTLTLDNQNMVITKARQNKWETGNNLDVLTYTNPSYPNLDHQRSVLFINKKYFLVIDRAIGEATGNLGVHWQLKEDSNPVFDKTKNRVYTTYRDGNNLMIQSLNADRTSLNEEEGKVSYVYNKELKRPAFVFEKPKKNAGTQNFVSIVYPYDGQKAPEISIRENKGNDFEKGKLNLTLTINGKQQLVLVP

Approximately 74 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, pH 7.4, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.