1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins Receptor Proteins Enzymes & Regulators
  3. EGF Superfamily ErbB3/HER3 Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. EGFR/ErbB family
  5. HER3 蛋白, Mouse (HEK293, His)

HER3 蛋白, Mouse (HEK293, His)

目录号: HY-P70857
产品使用指南

HER3 蛋白是一种重要的酪氨酸蛋白激酶和神经调节蛋白细胞表面受体,在与神经调节蛋白-1 (NRG1) 结合后激活,增强酪氨酸残基的磷酸化并与磷脂酰肌醇 3-激酶的 p85 亚基结合。它还被 CSPG5 激活,在调节骨髓细胞分化中发挥至关重要的作用。HER3 蛋白, Mouse (HEK293, His) 是重组的 HER3 蛋白,由 HEK293 表达,带有 C-6*His 标签。HER3 蛋白, Mouse (HEK293, His) 全长 622 个氨基酸,分子量为 90-120 kDa。

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描述

HER3 Protein, a vital tyrosine-protein kinase and neuregulin cell surface receptor, activates upon binding to neuregulin-1 (NRG1), enhancing phosphorylation on tyrosine residues and associating with the p85 subunit of phosphatidylinositol 3-kinase. It is also activated by CSPG5 and plays a crucial role in regulating myeloid cell differentiation. HER3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived HER3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HER3 Protein, Mouse (HEK293, His) is 622 a.a., with molecular weight of 90-120 kDa.

研究背景

HER3 蛋白是一种重要的酪氨酸蛋白激酶,充当神经调节蛋白的细胞表面受体。结合神经调节蛋白-1 (NRG1) 后,它会被激活,导致酪氨酸残基磷酸化增加,并随后与磷脂酰肌醇 3-激酶的 p85 亚基结合。此外,HER3 蛋白可以被 CSPG5 激活。值得注意的是,它在调节骨髓细胞分化中发挥重要作用。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q61526 (S20-H641)

基因 ID
蛋白结构
N-term
HER3 (S20-H641)
Accession # Q61526
6*His
C-term
同用名
Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3; HER3
氨基酸序列

SEMGNSQAVCPGTLNGLSVTGDADNQYQTLYKLYEKCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSVLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRFTQLTEILLGGVYIEKNDKLCHMDTIDWRDIVRVPDAEIVVKNNGGNCPPCHEVCKGRCWGPGPEDCQILTKTICAPQCNGRCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPAPLVYNKLTFQLEPNPHIKYQYGGVCVASCPHNFVVDQTFCVRACPADKMEVDKNGLKMCEPCRGLCPKACEGTGSGSRYQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRVYISANQQLCYHHSLNWTRLLRGPAEERLDIKYNRPLGECVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSREGVCVTHCNVLQGEPREFVHEAHCFSCHPECQPMEGTSTCNGSGSDACARCAHFRDGPHCVNSCPHGILGAKGPIYKYPDAQNECRPCHENCTQGCKGPELQDCLGQAEVLMSKPH

分子量

90-120 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HER3 Protein, Mouse (HEK293, His)
目录号:
HY-P70857
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