HER4 蛋白, Human (HEK293, His)

HER4 is an important tyrosine protein kinase receptor for members of the neuregulin and EGF families that directs heart, central nervous system, and mammary gland development. It is critical for myocardial differentiation, postnatal cardiomyocyte proliferation, neural crest cell migration, axon guidance, and mammary gland function. HER4 Protein, Human (HEK293, His) is the recombinant human-derived HER4 protein, expressed by HEK293 , with C-6*His labeled tag.

HER4是一种酪氨酸蛋白激酶，是神经调节蛋白和EGF家族成员的关键细胞表面受体，在心脏、中枢神经系统和乳腺的发育以及基因转录、细胞增殖、分化、迁移和凋亡。它对于胚胎发育和出生后心肌细胞增殖过程中正常的心肌分化至关重要。此外，HER4是胚胎中枢神经系统正常发育所必需的，特别是神经嵴细胞迁移和轴突引导，以及乳腺分化和泌乳诱导。作为神经调节蛋白 NRG1、NRG2、NRG3、NRG4 和 EGF 家族成员 BTC、EREG 和 HBEGF 的受体，配体结合触发受体二聚化和自磷酸化，产生细胞内磷酸酪氨酸的多种组合，从而引发配体特异性和环境特异性细胞反应。HER4 介导 SHC1 磷酸化并激活 MAP 激酶 MAPK1/ERK2 和 MAPK3/ERK1。同工型 JM-A CYT-1 和 JM-B CYT-1 磷酸化 PIK3R1，激活磷脂酰肌醇 3-激酶和 AKT1，以防止细胞凋亡并促进细胞响应 NRG1 迁移。同种型 JM-A CYT-2 和 JM-B CYT-2 缺乏 PIK3R1 相互作用所需的磷酸酪氨酸，因此放弃了这些作用。同工型 JM-A CYT-1 和 JM-A CYT-2 的蛋白水解加工产生可溶性细胞内结构域 (4ICD)，这些结构域易位至细胞核，促进 STAT5A 的核输入、乳腺上皮分化、细胞增殖和基因表达激活。此外，ERBB4 可溶性胞内结构域可以易位至线粒体，诱导细胞凋亡。

Immobilized Human HER4, at 2 μg/mL (100μL/well) can bind Biotinylated Human NRG1 β1, the ED₅₀ for this effect is 16.09 ng/mL.

Human

HEK293

C-6*His

Q15303-1 (Q26-R649)

2066  [NCBI]

QSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHAR

94-107 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.