HEXB/Hexosaminidase B 蛋白, Human (HEK293, His)

HEXB (hexosaminidase B) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycoconjugates, including oligosaccharides, glycolipids, and mucopolysaccharides. B isoenzymes efficiently hydrolyze neutral oligosaccharides, making them unique. HEXB/Hexosaminidase B Protein, Human (HEK293, His) is the recombinant human-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Human (HEK293, His) is 514 a.a., with molecular weight of 60-66 kDa.

HEXB（己糖胺酶 B）蛋白对各种糖复合物中发现的非还原端 N-乙酰基-D-己糖胺和/或硫酸化 N-乙酰基-D-己糖胺残基表现出水解活性，包括来自蛋白质、中性糖脂和特定糖脂的寡糖部分。粘多糖。值得注意的是，B 同工酶能够有效水解中性寡糖，使其有别于其他底物。虽然同工酶 A 在 GM2A 存在的情况下负责 GM2 神经节苷脂的降解，但同工酶 B 不参与该过程。在受精过程中，HEXB，特别是同工酶 B，在阻止多精受精的透明带中发挥着至关重要的作用。它位于未激活卵母细胞的皮质颗粒中，并在卵母细胞激活引发的皮质反应期间被胞吐。这种胞吐作用导致精子半乳糖基转移酶结合位点失活，有助于防止精子与透明带结合并确保多精卵透明带块的完整性。

Measured by its ability to hydrolyze 4methylumbelliferylNacetylβDglucosaminide (4MUGlcNAc) and the specific activity is > 3000 pmols/min/μg.

Human

HEK293

C-His

P07686 (A43-M556)

3074  [NCBI]

60-66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.