HGFA/HGF Activator 蛋白, Mouse (HEK293, His)

HGFA/HGF Activator Protein activates HGF by converting it to a heterodimeric form, crucial for cell growth, development, and tissue repair. It induces cellular responses like proliferation, migration, and differentiation. It regulates cell-matrix and cell-cell adhesion, morphology, and E-cadherin expression. It interacts with proteins including THSD1, PTK2/FAK1, TLN1, VCL, CTNNA1, APBB1IP, NRAP, CTNNB1, SYNM, SORBS1, and triggers conformational changes when binding to ACTN4, emphasizing its importance and therapeutic potential. HGFA/HGF Activator Protein, Mouse (HEK293, His) is the recombinant mouse-derived HGFA/HGF Activator protein, expressed by HEK293, with C-His, C-6*His labeled tag. The total length of HGFA/HGF Activator Protein, Mouse (HEK293, His) is 619 a.a., with molecular weight of approximately 107.5 kDa.

HGFA/HGF 激活蛋白负责通过将肝细胞生长因子 (HGF) 从单链转化为异二聚体形式来激活它。该异二聚体由通过二硫键连接在一起的短链和长链组成。HGFA/HGF 激活蛋白在各种生物过程中发挥着至关重要的作用，包括细胞生长、发育和组织修复。它对 HGF 的激活导致细胞反应的诱导，例如细胞增殖、迁移和分化。通过调节 HGF 活性，HGFA/HGF 激活蛋白有助于调节细胞-基质粘附、细胞-细胞粘附和细胞形态。它与多种蛋白质相互作用，包括 THSD1、PTK2/FAK1、TLN1 和 VCL，并且它与 CTNNA1 的关联对于其定位到细胞-细胞连接和 E-钙粘蛋白表达的调节至关重要。此外，HGFA/HGF激活蛋白与APBB1IP、NRAP、TLN1、CTNNB1、SYNM、SORBS1和CTNNA1形成复合物，并在与ACTN4结合时触发构象变化。其多方面的相互作用和活动凸显了其在细胞过程中的重要性，并强调了其潜在的治疗应用。

Measured in a cell proliferation assay using hepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.951 μg/mL, corresponding to a specific activity is 5.126×10³ U/mg.

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  Measured in a cell proliferation assay using hepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.951 μg/mL, corresponding to a specific activity is 5.126×10³ U/mg.

Mouse

HEK293

C-His;C-6*His

Q9R098 (Q35-S653)

54426  [NCBI]

QAGRNHTEPPGPNVTATPVTPTIPVISGNVSTSTESAPAAETEGPQSERYPPPSSSSPPGGQVLTESGQPCRFPFRYGGRMLHSCTSEGSAYRKWCATTHNYDRDRAWGYCAEVTLPVEGPAILDPCASGPCLNGGTCSSTHDHGSYHCSCPLAFTGKDCGTEKCFDETRYEYFEVGDHWARVSEGHVEQCGCMEGQARCEDTHHTACLSSPCLNGGTCHLIVGTGTSVCTCPLGYAGRFCNIVPTEHCFLGNGTEYRGVASTAASGLSCLAWNSDLLYQELHVDSVAAAVLLGLGPHAYCRNPDKDERPWCYVVKDNALSWEYCRLTACESLARVHSQTPEILAALPESAPAVRPTCGKRHKKRTFLRPRIIGGSSSLPGSHPWLAAIYIGNSFCAGSLVHTCWVVSAAHCFANSPPRDSITVVLGQHFFNRTTDVTQTFGIEKYVPYTLYSVFNPNNHDLVLIRLKKKGERCAVRSQFVQPICLPEAGSSFPTGHKCQIAGWGHMDENVSSYSNSLLEALVPLVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLVCEKNGVAYLYGIISWGDGCGRLNKPGVYTRVANYVDWINDRIRPPKRPVATS

approximately 107.5 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl, 2 mM CaCl₂, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.