HIFI Dna polymerase 蛋白, Thermococcus kodakarensis

HIFI DNA polymerase is known for its excellent processivity, rapid polymerization rate, and excellent DNA synthesis fidelity. It also has 3' to 5' exonuclease activity. Related to intein-encoded endonucleases, it suggests a potential role in intein mobility through site-specific recombination. HIFI Dna polymerase Protein, Thermococcus kodakarensis is the recombinant HIFI Dna polymerase protein, expressed by E. coli , with hFc labeled tag. HIFI Dna polymerase Protein, Thermococcus kodakarensis, has molecular weight of ~90 kDa.

HIFI DNA 聚合酶的特点是其卓越的持续合成能力、快速的聚合速率以及合成 DNA 的卓越保真度。除了聚合酶活性外，它还具有 3' 至 5' 核酸外切酶活性。值得注意的是，HIFI DNA 聚合酶与内含肽编码的核酸内切酶相关，这表明通过在缺乏内含肽的基因的“归巢位点”处由内切核酸酶裂解引发的位点特异性重组，HIFI DNA 聚合酶在内含肽移动性中具有潜在作用。在大肠杆菌中，PI-PkoI 识别最小序列 5'-GATTTTAGATCCCTGTACC-3' 并在 T-10 之后切割，而 PI-PkoII 识别 5'-CAGCTACTACGGTTAC-3' 并在 C-10 之后切割。该酶的功效，特别是在高细胞内 K(+) 环境中，强调了其在体内的潜在用途和活性。

Others

E. coli

hFc

P77933

3233723  [NCBI]

MASAILDTDYITEDGKPVIRIFKKENGEFKIEYDRTFEPYFYALLKDDSAIEEVKKITAERHGTVVTVKRVEKVQKKFLGRPVEVWKLYFTHPQDVPAIRDKIREHPAVIDIYEYDIPFAKRYLIDKGLVPMEGDEELKMLAFDIETLYHEGEEFAEGPILMISYADEEGARVITWKNVDLPYVDVVSTEREMIKRFLRVVKEKDPDVLITYNGDNFDFAYLKKRCEKLGINFALGRDGSEPKIQRMGDRFAVEVKGRIHFDLYPVIRRTINLPTYTLEAVYEAVFGQPKEKVYAEEITTAWETGENLERVARYSMEDAKVTYELGKEFLPMEAQLSRLVGQPLWDVSRSSTGNLVEWFLLRKAYERNEVAPNKPSEEEYQRRLRESYTGGFVKEPEKGLWENIVYLDFRALYPSIIITHNVSPDTLNLEGCKNYDIAPQVGHKFCKDIPGFIPSLLGHLLEERQKIKTKMKETQDPIEKILLDYRQKAIKLLANSFYGYYGYAKARWYCKECAESVTAWGRKYIELVWKELEEKFGFKVLYIDTDGLYATIPGGESEEIKKKALEFLKYINAKLPGALELEYEGFYKRGFFVTKKKYAVIDEEGKITTRGLEIVRRDWSEIAKETQARVLEALLKDGDVEKAVRIVKEVTEKLSKYEVPPEKLVIHEQITRDLKDYKATGPHVAVAKRLAARGVKIRPGTVISYIVLKGSGRIGDRAIPFDEFDPTKHKYDAEYYIENQVLPAVERILRAFGYRKEDLRYQKTRQVGLSAWLKPKGTLE

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.