1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. HIPK4 蛋白, Human (Sf9, GST)

HIPK4 蛋白, Human (Sf9, GST)

目录号: HY-P701700
产品使用指南

HIPK4蛋白是一种动态激酶,可以磷酸化人TP53的Ser-9位点,从而启动TP53抑制BIRC5启动子。这一关键作用表明它参与了 TP53 介导的细胞反应。HIPK4 蛋白, Human (Sf9, GST) 是重组的 HIPK4 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。HIPK4 蛋白, Human (Sf9, GST) 全长 615 个氨基酸。

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描述

HIPK4 protein is a dynamic kinase that can phosphorylate human TP53 at the Ser-9 site, thereby initiating TP53 to inhibit the BIRC5 promoter. This critical role suggests its involvement in TP53-mediated cellular responses. HIPK4 Protein, Human (Sf9, GST) is the recombinant human-derived HIPK4 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of HIPK4 Protein, Human (Sf9, GST) is 615 a.a., .

研究背景

HIPK4 蛋白是一种动态激酶,通过磷酸化人 TP53 Ser-9 从而在细胞调节中发挥至关重要的作用,从而引发 TP53 对 BIRC5 启动子的抑制。这表明 TP53 介导的细胞反应的复杂网络具有关键作用。此外,HIPK4 表现出作为转录因子辅阻遏物的潜力,表明对基因表达和转录调控具有更广泛的影响。HIPK4 在 TP53 磷酸化和潜在转录共抑制中的双重作用强调了其在调节关键细胞过程和促进基因表达调控景观中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

Q8NE63 (S2-H616)

基因 ID

147746

蛋白结构
N-term
GST
HIPK4 (S2-H616)
Accession # Q8NE63
C-term
同用名
HIPK4; Homeodomain-interacting protein kinase 4
纯度

Greater than 75% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HIPK4 Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HIPK4 Protein, Human (Sf9, GST)
目录号:
HY-P701700
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