p24 蛋白, HIV-1 (AAB50258, His)

The capsid protein p24 forms the conical core of the genome RNA-nucleocapsid complex in the virion. Promote immune invasion by hiding the viral DNA detected by CGAS. The viral capsid protein p24 is considered to be another early virological biomarker of infection. p24 Protein, HIV-1 (AAB50258, His) is the recombinant Virus-derived p24 protein, expressed by E. coli , with C-His labeled tag.

Gag- pol 多蛋白在病毒粒子组装过程中发挥核心作用，与 Gag 多蛋白合作协调基本事件，如与质膜结合，促进对球形颗粒形成至关重要的蛋白质-蛋白质相互作用，招募病毒 Env 蛋白，以及通过与 RNA包装序列 (Psi) 的直接相互作用包装基因组 RNA。衣壳蛋白 p24 在病毒粒子中形成包封基因组 RNA -核衣壳复合物的锥形核心。大多数核心是圆锥形的，只有 7% 是管状的。核心由衣壳蛋白六聚体亚基组成。核心在病毒粒子进入后很快被分解。衣壳通过隐藏 CGAS 检测的病毒 DNA 来促进免疫入侵。宿主限制因子如 TRIM5-α 或 TRIMCyp 结合逆转录病毒衣壳并导致衣壳过早解体，导致逆转录阻滞。TRIM5 衣壳限制是限制 HIV-1 进入人类的因素之一。宿主 PIN1 明显促进病毒粒子脱壳。另一方面，与 PDZD8 或 CYPA 的相互作用稳定衣壳。

Virus

E. coli

C-His

P04591-1/AAB50258 (P133-L363)

155030  [NCBI]

PIVQNIQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVL

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.