Envelope glycoprotein gp120 蛋白, HIV (Q9DKG6, HEK293, His)

The envelope glycoprotein gp120 protein facilitates viral capture and dissemination by dendritic cells and endothelial cells by attaching the virus to host lymphocytes through binding to the CD4 receptor. HIV exploits the migratory properties of dendritic cells to access CD4+ T cells, leading to infection. Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is the recombinant Virus-derived Envelope glycoprotein gp120 protein, expressed by HEK293 , with C-8*His labeled tag. The total length of Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is 498 a.a., with molecular weight of 105-130 kDa.

包膜糖蛋白 gp160 在宿主内质网中主要发生寡聚化成三聚体，然后转运至宿主高尔基体完成糖基化。随后，gp160 在反式高尔基体中被细胞弗林蛋白酶或类弗林蛋白酶蛋白酶裂解，导致 gp120 和 gp41 的激活和生成。表面蛋白 gp120 作为病毒生命周期中的关键介质，最初通过与主要受体 CD4 结合将病毒附着到宿主淋巴细胞上。这种相互作用诱导结构重排，为趋化因子辅助受体（例如 CXCR4 和/或 CCR5）创建高亲和力结合位点。此外，gp120 作为树突状细胞 (DC) 上的 CD209/DC-SIGN 和内皮细胞上的 CLEC4M/DC-SIGNR 的配体，促进病毒在粘膜表面捕获并随后传播到允许的细胞。这种与 DC 的相互作用使得 HIV 能够利用树突状细胞的迁移特性，从而接触到淋巴结中的 CD4+ T 细胞。病毒向 T 细胞的传播可以发生反式（病毒捕获和传播）或顺式（生产性 DC 感染后），从而导致强烈的感染。在反式感染中，与树突状细胞结合的病毒体在数天内仍保持感染性，可能在树突状细胞内的非溶酶体酸性细胞器中受到保护，从而增强了病毒从外周迁移到淋巴组织期间的感染潜力。到达淋巴组织后，完整的病毒粒子循环回到 DC 细胞表面，促进病毒传播至 CD4+ T 细胞。

Measured  in a cell proliferation assay using U87 human glioma cell. The  ED₅₀ this effect is 82.60 ng/mL, corresponding to a specific activity  is 1.211×10⁴ units/mg.

Virus

HEK293

C-8*His

Q9DKG6 (M1-E498)

/

MRVKETQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWRNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANWTNSNNTTNGPNKIGNITDEVKNCTFNMTTELKDKKQKVHALFYKLDIVQINSSEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNNTRTSITMGPGQVFYRTGDIIGDIRKAYCEINGIKWNEVLVQVTGKLKEHFNKTIIFQPPSGGDLEIITHHFSCRGEFFYCNTTKLFNNTCIGNTSMEGCNNTIILPCKIKQIINMWQGVGQAMYAPPISGRINCVSNITGILLTRDGGADNNTTNETFRPGGGNIKDNWRSELYKYKVVEIEPLGIAPTRAKRRVVE

105-130 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.