HLA-DPB1 蛋白, Human (His)

The HLA-DPB1 protein is an integral part of the immune system, binding antigens in the endocytic pathway of antigen-presenting cells (APCs). It presents the peptide on the cell surface for recognition by CD4 T cells. HLA-DPB1 Protein, Human (His) is the recombinant human-derived HLA-DPB1 protein, expressed by E. coli , with N-His labeled tag. The total length of HLA-DPB1 Protein, Human (His) is 194 a.a., with molecular weight of ~26.8 kDa.

HLA-DPB1 蛋白通过结合抗原呈递细胞 (APC) 内吞途径中的抗原衍生肽并将其呈递到细胞表面以供 CD4 T 细胞识别，从而在免疫系统中发挥关键作用。HLA-DPB1 的肽结合裂隙可容纳 10 至 30 个残基的肽，主要通过进入内吞途径的蛋白质降解产生。这种外源抗原呈递途径涉及溶酶体蛋白酶和其他水解酶处理 APC 吸收的抗原。值得注意的是，胃肠道细胞，包括上皮细胞，表达 MHC II 类分子和 CD74，充当非常规 APC。功能性 MHC II 类分子的组装涉及三个 MHC II 类分子与内质网 (ER) 中的 CD74 三聚体的结合，形成异源单体。进入内体/溶酶体系统后，CD74 经历连续降解，在每个 MHC II 类分子上留下称为 CLIP 的片段。HLA-DM 促进 CLIP 去除，稳定 II 类 MHC，直到高亲和力抗原肽结合。HLA-DO 调节 B 细胞中 HLA-DM 和 MHC II 类之间的相互作用，溶酶体酸化影响有效的肽装载。MHC II 类分子与肽结合，然后被转运到细胞膜表面进行免疫识别。

Human

E. coli

N-His

P04440 (R30-R223)

3115  [NCBI]

RATPENYLFQGRQECYAFNGTQRFLERYIYNREEFARFDSDVGEFRAVTELGRPAAEYWNSQKDILEEKRAVPDRMCRHNYELGGPMTLQRRVQPRVNVSPSKKGPLQHHNLLVCHVTDFYPGSIQVRWFLNGQEETAGVVSTNLIRNGDWTFQILVMLEMTPQQGDVYTCQVEHTSLDSPVTVEWKAQSDSAR

Approximately 26.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.