HMGB1/HMG-1 蛋白, Human (HEK293, Fc)

The multifunctional and redox-sensitive HMGB1/HMG-1 protein plays multiple roles in cellular compartments. In the nucleus, it serves as a major chromatin-associated non-histone protein involved in key processes such as replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. HMGB1/HMG-1 Protein, Human (HEK293, Fc) is the recombinant human-derived HMGB1/HMG-1 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of HMGB1/HMG-1 Protein, Human (HEK293, Fc) is 215 a.a., with molecular weight of ~59.78 kDa.

HMGB1/HMG-1 是一种多功能氧化还原敏感蛋白，在不同的细胞区室中表现出不同的作用。在细胞核内，它是一种主要的染色质相关非组蛋白，作为 DNA 伴侣参与复制、转录、染色质重塑、V(D)J 重组、DNA 修复和基因组稳定性等关键过程。HMGB1/HMG-1 被提议作为核酸的通用生物传感器，在促进宿主对无菌和感染信号的炎症反应、协调先天性和适应性免疫反应方面发挥着关键作用。在细胞质中，它充当免疫原性核酸的传感器和/或伴侣，激活 TLR9 介导的免疫反应并介导自噬。HMGB1/HMG-1 释放到细胞外环境，与 DNA、核小体、IL-1 beta、CXCL12、AGER 异构体 2/sRAGE、脂多糖 (LPS) 和脂磷壁酸 (LTA) 等多种分子结合，通过多种方式激活细胞表面受体。细胞外HMGB1以不同的氧化还原状态存在，完全还原的HMGB1作为趋化因子，二硫键HMGB1作为细胞因子，磺酰基HMGB1促进免疫耐受。除了其免疫调节作用之外，HMGB1/HMG-1 还涉及促血管生成活性、血小板活化、通过 RAGE 的神经元生长信号传导，以及可能参与扩展的聚谷氨酰胺蛋白的积累。其核功能归因于完全减少的 HMGB1，包括与染色质关联、DNA 弯曲以及通过成环增强 DNA 灵活性，促进各种基因启动子活性。此外，HMGB1/HMG-1 可能在核苷酸切除修复、错配修复、碱基切除修复、双链断裂修复、V(D)J 重组、组蛋白 H1 置换、重组核小体、增强转录因子结合和调节中发挥作用。端粒酶复合物。HMGB1/HMG-1 的复杂功能凸显了其在多种细胞过程中的重要性。

1. Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ for this effect is 2.196-3.920 μg/mL.
2. Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse AGER at 2 μg/ml (100 μl/well) can bind human HMGB1. The EC₅₀ of human HMGB1 is 0.23 μg/mL.

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  Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED₅₀ for this effect is 3.920 μg/mL, corresponding to a specific activity is 255.102 U/mg.

Human

HEK293

N-hFc

P09429/NP_002119.1 (M1-E215)

3146  [NCBI]

MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE

Approximately 58-59.78 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.