HNRNPA1 蛋白, Human (His)

The HNRNPA1 protein is complexly involved in multiple RNA processing functions, packaging pre-mRNA into hnRNP particles, promoting Poly(A) mRNA transport, and regulating splice site selection. Crucially, it binds inhibitoryly to sequences flanking PKM exon 9, favoring the inclusion of exon 10 in pyruvate kinase PKM splicing, thereby generating the PKM M2 isoform. HNRNPA1 Protein, Human (His) is the recombinant human-derived HNRNPA1 protein, expressed by E. coli , with N-6*His labeled tag.

HNRNPA1 蛋白错综复杂地参与 RNA 加工的各个方面，包括将前 mRNA 包装到 hnRNP 颗粒中、将 Poly(A) mRNA 从细胞核转运到细胞质，以及剪接位点选择的调节。值得注意的是，它通过抑制性地结合 PKM 外显子 9 侧翼的序列，抑制外显子 9 包含并有利于外显子 10 包含，从而在丙酮酸激酶 PKM 的剪接中发挥关键作用，最终导致 PKM M2 同工型的产生。此外，HNRNPA1 表现出与内部核糖体进入位点 (IRES) 结合的能力，从而抑制凋亡蛋白酶激活因子 APAF1 的翻译。此外，它还可以与特定的 miRNA 发夹结合，扩大其在转录后基因调控中的作用。在微生物感染的条件下，HNRNPA1 也可能在 HCV RNA 复制中发挥作用，突显其在促进 RNA 代谢和病毒过程的各个方面的多功能性。

Human

E. coli

N-6*His

P09651-1 (S2-Q354)

3178  [NCBI]

SKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQ

Approximately 40.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.