HNRNPH1 蛋白, Human (His-SUMO)

The HNRNPH1 protein is an important component of the hnRNP complex and plays a key role in pre-mRNA processing events leading to mRNA conversion in the cytoplasm. Functionally, it cooperates with CUGBP1 to regulate alternative splicing of pre-mRNA, specifically inhibiting the inclusion of insulin receptor (IR) pre-mRNA exon 11 in myoblasts. HNRNPH1 Protein, Human (His-SUMO) is the recombinant human-derived HNRNPH1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HNRNPH1 Protein, Human (His-SUMO) is 448 a.a., with molecular weight of ~65.1 kDa.

HNRNPH1 蛋白作为异质核核糖核蛋白 (hnRNP) 复合物的组成部分发挥着至关重要的作用，该复合物是前 mRNA 在细胞质中功能性转化为可翻译 mRNA 之前的加工事件中的关键实体。从功能上讲，HNRNPH1 介导前体 mRNA 选择性剪接的调节，对成肌细胞中胰岛素受体 (IR) 前体 mRNA 外显子 11 的包含具有显着的抑制作用，这是它与 CUGBP1 合作完成的过程。HNRNPH1 对 IR RNA 和聚 (RG) 具有特异性结合亲和力，并且它与 FUBP2、PTBP1 和 PTBP2 一起形成三元复合物的一部分。此外，HNRNPH1 在剪接体 C 复合物中被识别，并与 IGF2BP1、CUGBP1（以 RNA 依赖性方式）和 MBNL1（以 RNA 独立方式）相互作用。这些相互作用强调了 HNRNPH1 在协调前 mRNA 加工和选择性剪接中的多方面和动态作用，有助于基因表达的复杂调节。

Human

E. coli

N-6*His;N-SUMO

P31943 (M2-A449)

3187  [NCBI]

MLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

Approximately 65.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.