1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. HELPY 蛋白, Helicobacter pylori (His)

HELPY 蛋白, Helicobacter pylori (His)

目录号: HY-P702130
产品使用指南

HpGT 蛋白,幽门螺杆菌是一种谷胱甘肽降解酶,是感染的毒力因子。谷胱甘肽水解酶原属于γ-谷氨酰转移酶家族。幽门螺杆菌-谷氨酰转肽酶表达为 60-kDa 无活性前体,必须经过自催化处理才能生成具有完整 γ-谷氨酰酰胺键水解酶活性的 40-kDa/20-kDa 异二聚体。HELPY 蛋白, Helicobacter pylori (His) 是重组的 HpGT 蛋白,由 E. coli 表达,带有 N-6*His 标签。HELPY 蛋白, Helicobacter pylori (His) 全长 543 个氨基酸。

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描述

HpGT Protein, Helicobacter pylori is a glutathione-degrading enzyme that is a virulence factor in infection. Glutathione hydrolase proenzyme belongs to the gamma-glutamyltransferase family. pylorigamma-glutamyltranspeptidase is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity[1][2]. HELPY Protein, Helicobacter pylori (His) is the recombinant HpGT protein, expressed by E. coli , with N-6*His labeled tag. The total length of HELPY Protein, Helicobacter pylori (His) is 543 a.a., .

研究背景

幽门螺杆菌-谷氨酰转肽酶 (HpGT) 表达为 60-kDa 无活性前体,必须经过自催化处理才能生成具有完整 γ-谷氨酰酰胺键水解酶活性的 40-kDa/20-kDa 异二聚体。所得 N 末端 Thr-380 已被证明是自动加工和酶促反应中的催化亲核试剂。HpGT 是 N 端亲核水解酶超家族的成员。HPGT 还被证明可以上调人胃粘膜细胞中的 COX-2 和表皮生长因子相关肽,并诱导人胃上皮细胞凋亡[1]
幽门螺杆菌 γ-谷氨酰转肽酶 (HpGT) 催化谷胱甘肽降解的第一步,通过 γ-谷氨酰酶中间体从三肽及其缀合物中去除谷氨酸。γ-谷氨酰基可以转移至水(水解)或氨基酸或短肽(转肽作用)[2]

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

O25743 (S25-F567, T380A)

基因 ID

/

蛋白结构
N-term
6*His
HpGT (S25-F567, T380A)
Accession # O25743
C-term
同用名
Glutathione hydrolase proenzyme
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HELPY Protein, Helicobacter pylori (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HELPY Protein, Helicobacter pylori (His)
目录号:
HY-P702130
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