1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. HELPY 蛋白, Helicobacter pylori (T380A)

HELPY 蛋白, Helicobacter pylori (T380A)

目录号: HY-P702129
产品使用指南 技术支持

HpGT 蛋白,幽门螺杆菌是一种谷胱甘肽降解酶,是感染的毒力因子。谷胱甘肽水解酶原属于γ-谷氨酰转移酶家族。幽门螺杆菌-谷氨酰转肽酶表达为 60-kDa 无活性前体,必须经过自催化处理才能生成具有完整 γ-谷氨酰酰胺键水解酶活性的 40-kDa/20-kDa 异二聚体。HELPY 蛋白, Helicobacter pylori (T380A) 是重组的 幽门螺杆菌 HpGT 蛋白,由 E. coli 表达,不带标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

HpGT Protein, Helicobacter pylori is a glutathione-degrading enzyme that is a virulence factor in infection. Glutathione hydrolase proenzyme belongs to the gamma-glutamyltransferase family. pylorigamma-glutamyltranspeptidase is expressed as a 60-kDa inactive precursor that must undergo autocatalytic processing to generate a 40-kDa/20-kDa heterodimer with full gamma-glutamyl amide bond hydrolase activity[1][2]. HELPY Protein, Helicobacter pylori (T380A) is the recombinant HpGT protein, expressed by E. coli , with tag free.

研究背景

幽门螺杆菌-谷氨酰转肽酶 (HpGT) 表达为 60-kDa 无活性前体,必须经过自催化处理才能生成具有完整 γ-谷氨酰酰胺键水解酶活性的 40-kDa/20-kDa 异二聚体。所得 N 末端 Thr-380 已被证明是自动加工和酶促反应中的催化亲核试剂。HpGT 是 N 端亲核水解酶超家族的成员。HPGT 还被证明可以上调人胃粘膜细胞中的 COX-2 和表皮生长因子相关肽,并诱导人胃上皮细胞凋亡[1]
幽门螺杆菌 γ-谷氨酰转肽酶 (HpGT) 催化谷胱甘肽降解的第一步,通过 γ-谷氨酰酶中间体从三肽及其缀合物中去除谷氨酸。γ-谷氨酰基可以转移至水(水解)或氨基酸或短肽(转肽作用)[2]

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

O25743 (S25-F567, T380A)

基因 ID

/

蛋白结构
N-term
HpGT (S25-F567, T380A)
Accession # O25743
C-term
同用名
Glutathione hydrolase proenzyme
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HELPY Protein, Helicobacter pylori (T380A) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
HELPY Protein, Helicobacter pylori (T380A)
目录号:
HY-P702129
需求量: