HSF1 蛋白, Human (His)

HSF1, a stress-inducible transcription factor, orchestrates the heat shock response by activating heat shock genes through binding to heat shock elements (HSEs). In unstressed cells, it remains inactive in a multichaperone complex. Upon stress, HSF1 undergoes homotrimerization, binds to HSEs, and activates HSP gene transcription. It also participates in various cellular functions, including regulation of mitotic progression, repression of c-fos gene, and negative regulation of non-homologous end joining repair. Additionally, HSF1 plays a role in HIV-1 transcriptional reactivation. HSF1 Protein, Human (His) is the recombinant human-derived HSF1 protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of HSF1 Protein, Human (His) is 528 a.a., with molecular weight of ~67 kDa.

HSF1 是一种应激反应性 DNA 结合转录因子，是热休克反应 (HSR) 的中心协调者，可导致多种热休克蛋白 (HSP) 的转录激活，这些热休克蛋白对于细胞保护免受损伤至关重要。在无应激条件下，HSF1 驻留在含有 HSP90 的多伴侣复合物中，保持失活的单体状态。当暴露于压力，特别是热时，HSF1 发生同源三聚化，并通过与启动子中的热休克元件 (HSE) 结合来激活 HSP 基因的转录。这种转变是可逆的，并且在 HSR 的恢复阶段，HSF1 返回到其未激活的形式。除了转录激活之外，HSF1 还参与各种细胞过程，包括与 TTC5/STRAP 和 p300/EP300 形成染色质相关复合物、抑制 Ras 诱导的 c-fos 基因转录、调节前 mRNA 加工、应激诱导的核输出mRNA 和有丝分裂进展的调节。此外，在微生物感染的情况下，HSF1 通过与 HIV-1 长末端重复启动子结合，在潜在人类免疫缺陷病毒 (HIV-1) 转录的重新激活中发挥作用。

Human

E. coli

N-His;N-6*His

Q00613-1 (D2-S529)

3297  [NCBI]

DLPVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTKLLTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIPLMLNDSGSAHSMPKYSRQFSLEHVHGSGPYSAPSPAYSSSSLYAPDAVASSGPIISDITELAPASPMASPGGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTDARGHTDTEGRPPSPPPTSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLSSHGFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISLLTGSEPPKAKDPTVS

Approximately 67 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.