ICAM-3/CD50 蛋白, Human (HEK293, His)

The ICAM-3/CD50 protein acts as a ligand for the leukocyte adhesion protein LFA-1 and integrin α-D/β-2, suggesting involvement in cell adhesion. ICAM-3 cooperates with integrin α-L/β-2 to facilitate phagocytosis of apoptotic neutrophils, suggesting a role in immune response regulation and apoptotic cell clearance. ICAM-3/CD50 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-3/CD50 protein, expressed by HEK293 , with C-His labeled tag. The total length of ICAM-3/CD50 Protein, Human (HEK293, His) is 456 a.a., with molecular weight of 100-120 kDa.

ICAM-3/CD50 蛋白作为白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）和整合素 α-D/β-2 的配体，表明其参与细胞粘附过程。当与整合素 α-L/β-2 结合时，ICAM-3 在巨噬细胞对凋亡中性粒细胞的吞噬作用中发挥重要作用，表明其在调节免疫反应和清除凋亡细胞中具有潜在作用。此外，ICAM-3 与 moesin/MSN 相互作用，表明它参与了可能影响细胞信号传导或结构组织的蛋白质-蛋白质相互作用。ICAM-3 的多方面相互作用强调了它在细胞粘附和免疫相关过程的复杂网络中的重要性。

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated jurkatcells. When 5x10⁴ cells/well are added to rhICAM-3/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well) in the presence of 20 ng/mL PMA, approximately 64.39% will adhere after 1 hourat 37°C.

Human

HEK293

C-His

P32942 (Q30-H485)

3385  [NCBI]

QEFLLRVEPQNPVLSAGGSLFVNCSTDCPSSEKIALETSLSKELVASGMGWAAFNLSNVTGNSRILCSVYCNGSQITGSSNITVYRLPERVELAPLPPWQPVGQNFTLRCQVEDGSPRTSLTVVLLRWEEELSRQPAVEEPAEVTATVLASRDDHGAPFSCRTELDMQPQGLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSH

100-120 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5 or pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.