IFN-gamma 蛋白, Cynomolgus (HEK293, His)

IFN-gamma (interferon-gamma) is a type II interferon derived from immune cells such as T cells and NK cells, which can significantly activate effector immune cells and enhance antigen presentation. It affects gene regulation through the JAK-STAT pathway and its receptor IFNGR1. IFN-gamma Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IFN-gamma protein, expressed by HEK293 , with C-His labeled tag. The total length of IFN-gamma Protein, Cynomolgus (HEK293, His) is 142 a.a., with molecular weight of 25-35 kDa.

IFN-gamma（干扰素-gamma）是一种由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥关键作用。其主要信号通路涉及与其受体IFNGR1相互作用后的JAK-STAT通路，影响基因调控。IFN-γ 结合后，IFNGR1 胞内结构域打开，允许下游信号成分 JAK2、JAK1 和 STAT1 关联，从而导致 STAT1 激活、核转位和 IFN-γ 调节基因的转录。许多这些诱导基因是转录因子，例如 IRF1，能够进一步驱动后续转录波的调节。IFN-γ 通过诱导免疫蛋白酶体亚基替换催化蛋白酶体亚基，从而促进 I 类抗原呈递途径，从而增加 I 类 MHC 负载的肽的数量、质量和库。它还通过诱导激活剂 PA28 的表达来提高肽生成的效率，PA28 与蛋白酶体结合并改变其蛋白水解切割偏好。此外，IFN-γ 通过促进组织蛋白酶 B/CTSB、H/CTSH 和 L/CTSL 等关键分子的表达，上调细胞表面的 MHC II 复合物。除了其直接的免疫功能外，IFN-γ还参与造血干细胞在发育过程中和稳态条件下的调节，影响其发育、静止和分化。IFN-γ 作为同二聚体存在，通过其胞外结构域与 IFNGR1 相互作用；这种相互作用对于促进 IFNGR1 二聚化以及协调 IFN-γ 在免疫反应和造血中的多样化和关键功能至关重要。

Immobilized Cynomolgus IFN gamma, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC₅₀ of 39.4 ng/mL determined by ELISA.

Cynomolgus

HEK293

C-His

P63309 (Q24-Q165)

102128291  [NCBI]

25-35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.