IFN-gamma 蛋白, Human (HEK293)

IFN-gamma Protein is a dimeric soluble cytokine. It exerts antibacterial, antiviral and antitumor effects through JAK-STAT, mTOR, MAPK and PI3K/AKT signaling pathways. IFN-gamma Protein, Human (HEK293) is the recombinant human-derived IFN-gamma protein, expressed by HEK293 , with tag free. The total length of IFN-gamma Protein, Human (HEK293) is 143 a.a., with molecular weight of 20-25 & 16-17 kDa, respectively.

IFN-gamma 是一种二聚体可溶性细胞因子，是II 型干扰素中唯一的成员。IFN-gamma 由免疫细胞T 细胞和NK 细胞产生，通过激活效应免疫细胞和增强抗原呈递，在抗菌、抗病毒和抗肿瘤反应中发挥重要作用。IFN-gamma 通过JAK-STAT 通路与其受体IFNGR1 相互作用后影响基因调控，也可触发mTOR、MAPK 和PI3K/AKT信号通路。IFN-gamma 通过诱导催化蛋白酶体亚基替换为免疫蛋白酶体亚基，在I 类抗原递呈途径中发挥作用。IFN-gamma 通过促进组织蛋白酶B/CTSB、H/CTSH 和L/CTSL 等几个关键分子的表达，上调细胞表面的MHC II 复合物。IFN-gamma 通过影响造血干细胞的发育、静止和分化，参与造血干细胞在发育和稳态条件下的调控。

Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells.The ED₅₀ for this effect is <0.3176 ng/mL, corresponding to a specific activity is >3.148×10⁶ Unit/mg.

• 
  Measured by its ability to inhibit the proliferation of HT-29 cells. The ED₅₀ for this effect is 0.1895 ng/mL, corresponding to a specific activity is 5.277×10⁶ Unit/mg.

Human

HEK293

Tag Free

P01579 (Q24-Q166)

3458  [NCBI]

QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

20-25 & 16-17kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 4% Mannitol, 2% Sucrose, 0.02% Tween80, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.