IFN-gamma R1/CD119 蛋白, Human (HEK293, hFc)

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate^[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)^[2]. IFN-gamma R1/CD119 Protein, Human (HEK293, hFc) is a recombinant human IFN-gamma R1 (E18-G245) with C-terminal hFc tag, which is produced in HEK293 cells.

IFN-γ R1 (CD119) 是 IFN-γ 受体的亚基之一，是 IFN-γ 的受体。IFN-γ R1 组成型表达于几乎所有细胞的表面^[1]。
IFN-γ R1 可以与 IFN-γ R2 结合形成功能性受体。与 IFN-γ 结合后，IFNγR1 和 IFNγR2 寡聚并转磷酸化^[1]。然后，JAK1 和 JAK2 被磷酸化并激活，STAT1 被招募到受体复合物中。IFNγR1 的磷酸化为 STAT1 创建了一个对接位点，并导致 STAT1 磷酸化。磷酸化的 STAT1 易位到细胞核，在那里调节 IFN 反应基因（例如 CD54）的表达。编码 IFN-gamma R1 的 IFNGR1 基因突变会导致原发性免疫缺陷并导致分枝杆菌感染，例如孟德尔分枝杆菌病易感性 (MSMD)^[2]
人类 IFN-gamma R1 由胞外结构域 (E18-G245)、螺旋结构域 (S246-I266) 和胞质结构域 (C267-S489) 组成。IFNAR1的氨基酸序列在不同物种中有所不同。人 IFN-γ R1 与小鼠 IFN-γ R1 的氨基酸序列有 50% 的同一性。IFN-gamma R1 在抗菌、抗病毒和抗肿瘤反应中发挥着关键作用^[2]。

Immobilized Human Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC₅₀ of 32.7ng/ml determined by ELISA.

Human

HEK293

C-hFc

P15260-1/AAH05333 (E18-G245)

3459  [NCBI]

60-75 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Castro F, et al. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018 May 4;9:847.  [Content Brief]

  [2]. van de Vosse E, et al. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database. Hum Mutat. 2017 Oct;38(10):1286-1296.  [Content Brief]