IFN-lambda 2/IL-28A 蛋白, Mouse (HEK293, N-His)

IFN-lambda 2/IL-28A protein is a cytokine with antiviral, antitumor, and immunomodulatory activities. It plays a key role in the antiviral defense of epithelial tissues by binding to IL10RB and IFNLR1 receptor complexes, activating the JAK/STAT signaling pathway. IFN-lambda 2/IL-28A Protein, Mouse (HEK293, N-His) is the recombinant mouse-derived IFN-lambda 2/IL-28A protein, expressed by HEK293 , with N-6*His labeled tag. The total length of IFN-lambda 2/IL-28A Protein, Mouse (HEK293, N-His) is 174 a.a., with molecular weight of ~27 kDa.

IFN-lambda 2/IL-28A 蛋白是一种多功能细胞因子，具有抗病毒、抗肿瘤和免疫调节活性，在抗病毒宿主防御（主要在上皮组织内）中发挥着至关重要的作用。作为由 IL10RB 和 IFNLR1 组成的异二聚体 II 类细胞因子受体的配体，其受体参与启动 JAK/STAT 信号通路，导致 IFN 刺激基因 (ISG) 的表达，从而建立抗病毒状态。由于受体分布有限，由于其受体 IFNLR1 的上皮细胞特异性表达，它主要在上皮细胞中发挥作用。虽然它不被认为对于阴道感染中早期病毒激活的宿主防御至关重要，但它在 Toll 样受体 (TLR) 诱导的抗病毒防御中发挥着重要作用，并且在肠上皮的抗病毒免疫防御中发挥着至关重要的作用。此外，IFN-lambda 2/IL-28A 通过上调 MHC I 类抗原表达发挥免疫调节作用，从而影响免疫反应。

Measured in a cell inhibitor assay using A549 cells. The ED₅₀ for this effect is 2.972 ng/mL, corresponding to a specific activity is 3.365×10⁵ units/mg.

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  Measured in a cell inhibitor assay using A549 cells. The ED₅₀ for this effect is 2.972 ng/mL , corresponding to a specific activity is 3.365×10⁵ units/mg.

Mouse

HEK293

N-6*His

Q4VK74 (D20-193V)

330496

DPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKDAIEKRLLEKDLRCSSHLFPRAWDLKQLQVQERPKALQAEVALTLKVWENMTDSALATILGQPLHTLSHIHSQLQTCTQLQATAEPRSPSRRLSRWLHRLQEAQSKETPGCLEASVTSNLFRLLTRDLKCVANGDQCV

Approximately 27 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.