IGFBP-5 蛋白, Human

IGFBP-5 Protein, Human is a unique insulin-like growth factor-binding protein, modulates the actions of secreted insulin-like growth factors, and stimulates osteoblast proliferation.

胰岛素样生长因子结合蛋白 (IGFBP) 通过与分泌的胰岛素样生长因子 (IGF) 结合来调节其作用，并通过以这种结合形式隔离 IGF 来延长 IGF 在细胞外环境和循环中的半衰期。IGFBP 增强或抑制 IGF 对靶细胞的作用；单个 IGFBP 可以抑制或增强 IGF 对骨中成骨细胞的作用。人胰岛素样生长因子结合蛋白 5 (rhIGFBP-5) 是骨骼中储存的一种独特且最丰富的 IGFBP，对羟基磷灰石和细胞外基质蛋白具有高特异性结合亲和力，因此将其及其结合的 IGF 固定在骨骼内。rhIGFBP-5 在体外持续刺激成骨细胞增殖，从而增加成骨细胞的数量^[1]。

Human

CHO

C-6*His

P24593 (L21-E272)

3488  [NCBI]

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

30-40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150mM NaCl, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705.  [Content Brief]