IGFBP-2 蛋白, Mouse (HEK293, His)

IGFBP-2 protein, a member of the IGF-binding protein family, inhibits IGF-mediated growth and developmental rates. It can extend the half-life of IGF and exhibit dual functions by inhibiting or stimulating the growth-promoting effect of IGF in cell culture. IGFBP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-2 Protein, Mouse (HEK293, His) is 271 a.a., with molecular weight of ~32-40 kDa.

IGFBP-2 蛋白充当抑制剂，专门针对 IGF 介导的生长和发育速率。作为 IGF 结合蛋白家族的一员，IGFBP-2 可延长 IGF 的半衰期，并具有双重功能，即抑制或刺激细胞培养中 IGF 的生长促进作用。这种调节是通过改变 IGF 与其细胞表面受体之间的相互作用而发生的。值得注意的是，与 IGF1 相比，IGFBP-2 对 IGF2 具有更高的结合亲和力，强调了其在精细调节 IGF 信号通路复杂平衡中的作用。

Measured by its ability to inhibit the biological activity of IGF-I on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.2342 µg/mL in the presence of 30 ng/mL IGF-I, corresponding to a specific activity is 4.269×10³ units/mg.

Mouse

HEK293

C-His

P47877 (E35-Q305)

16008  [NCBI]

Approximately 32-40 kDa

Greater than 95% as determined by Tris-Bis PAGE

无菌粉末

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.