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  3. IGKC 蛋白, Human (Sf9, His)

IGKC 蛋白, Human (Sf9, His)

目录号: HY-P703484
产品使用指南

Ig kappa 蛋白是免疫球蛋白轻链的恒定区,是 B 淋巴细胞产生的膜结合或分泌糖蛋白的一部分。IGKC 蛋白, Human (Sf9, His) 是重组的 IGKC 蛋白,由 Sf9 insect cells 表达,带有 His 标签。IGKC 蛋白, Human (Sf9, His)

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描述

Ig kappa protein is the constant region of an immunoglobulin light chain and is part of a membrane-bound or secreted glycoprotein produced by B lymphocytes. IGKC Protein, Human (Sf9, His) is the recombinant human-derived IGKC, expressed by Sf9 insect cells , with His labeled tag. ,

研究背景

免疫球蛋白轻链恒定区(以 Ig kappa 蛋白为例)在抗体(也称为免疫球蛋白)的结构和功能中起着至关重要的作用。这些膜结合或分泌的糖蛋白由 B 淋巴细胞产生,在体液免疫识别阶段充当受体。结合特定抗原后,膜结合免疫球蛋白启动 B 淋巴细胞的克隆扩增和分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白反过来协调体液免疫的效应阶段,导致结合抗原的消除。抗原结合位点由一条重链的可变结构域与其相关轻链的可变结构域共同形成,导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变,从而在暴露于抗原和选择后允许亲和力成熟。免疫球蛋白的特征是两条相同的重链和两条相同的轻链,通过二硫键错综复杂地连接。

种属

Human

表达系统

Sf9 insect cells

标签

His

蛋白编号

P01834 (R1-G105, E16S)

基因 ID

/

同用名
Immunoglobulin kappa constant; Ig kappa chain C region; Ig kappa chain C region AG; Ig kappa chain C region CUM; Ig kappa chain C region EU; Ig kappa chain C region OU; Ig kappa chain C region ROY; Ig kappa chain C region TI; IGKC; Homo sapiens; Human
分子量

26.0 KDa; 23.3 KDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IGKC Protein, Human (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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