IHH 蛋白, Human

IHH Protein, Human is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification.

印度刺猬 (Ihh) 编码信号因子 Hedgehog 家族的成员，最初在早期软骨骨骼元件的软骨细胞中表达^[1]。Indian Hedgehog 是结肠上皮细胞分化过程中 Wnt 信号传导的拮抗剂^[2]。Ihh 参与软骨细胞分化、增殖和成熟，尤其是在软骨内骨化过程中。它通过甲状旁腺激素相关肽 (PTHrP) 的反馈控制来调节其作用^[3]。

1.The ED₅₀ is <3 μg/mL as measured by its ability to induce alkaline phosphatase production by CCL-226 cells.
2.Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts. The ED₅₀ for this effect is typically 5.587 μg/mL, corresponding to a specific activity is 179.0 units/mg.

• 
  Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts. The ED₅₀ for this effect is typically 5.587 μg/mL, corresponding to a specific activity is 179.0 units/mg.

Human

E. coli

Tag Free

Q14623 (G29-G202)

3549  [NCBI]

GPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGG

Approximately 20 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Van den Brink GR, et al. Indian Hedgehog is an antagonist of Wnt signaling in colonic epithelial cell differentiation. Nat Genet. 2004 Mar;36(3):277-82. Epub 2004 Feb 8.  [Content Brief]

  [2]. St-Jacques B, et al. Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation. Genes Dev. 1999 Aug 15;13(16):2072-86.  [Content Brief]

  [3]. Vortkamp A, et al. Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein. Science. 1996 Aug 2;273(5275):613-22.  [Content Brief]