IL-1 alpha 蛋白, Rhesus macaque

IL-1 alpha, a cytokine present in non-hematopoietic cells, links innate and adaptive immunity. Binding to its receptor IL1R1, IL-1 alpha forms a receptor complex, activating signaling pathways involving MYD88, IRAK1, and IRAK4. It activates NF-kappa-B and MAPK pathways. Released during cell death, IL-1 alpha induces inflammation, senses DNA damage, and interacts with TMED10, IL1R1, and S100A13 for secretion and plasma membrane translocation. IL-1 alpha Protein, Rhesus macaque is the recombinant Rhesus Macaque-derived IL-1 alpha protein, expressed by E. coli , with tag free. The total length of IL-1 alpha Protein, Rhesus macaque is 159 a.a., with molecular weight of ~18.1 kDa.

IL-1 α 蛋白是一种几乎所有静止非造血细胞中固有的细胞因子，在炎症中发挥着关键作用，是先天性免疫系统和适应性免疫系统之间的重要纽带。在与其受体 IL1R1 结合后，IL-1 α 与其辅助蛋白 IL1RAP 一起形成高亲和力白细胞介素 1 受体复合物，启动涉及招募接头分子（如 MYD88、IRAK1 或 IRAK4）的信号传导通路。这反过来又激活 NF-kappa-B 和三个 MAPK 通路——p38、p42/p44 和 JNK 通路。在内部，IL-1 α 充当警报素，在细胞死亡过程中细胞膜破裂后被释放到细胞外空间，从而诱导炎症并向宿主发出受伤或损害的信号。除了在细胞坏死期间释放危险信号之外，IL-1 α 还可以直接感知 DNA 损伤并作为基因毒性应激信号，而不会损害细胞完整性。作为单体，IL-1 α 与 TMED10 相互作用，促进其从细胞质易位至内质网-高尔基中间室 (ERGIC) 进行分泌。此外，它还与 IL1R1 和 S100A13 相互作用，后者是 IL-1 α 输出的第一步，随后该复合物直接易位穿过质膜。

1.Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 10 pg/mL, corresponding to a specific activity of >1.0x10⁸ IU/mg.
2.Measured in a cell proliferation assay using CTLL-2 cells. The ED₅₀ for this effect is 19.28 pg/mL, corresponding to a specific activity is 5.187×10⁷ U/mg.

Rhesus Macaque

E. coli

Tag Free

P48089 (S113-A271)

700193  [NCBI]

SAPFSFLSNMTYHFIRIIKHEFILNDTLNQTIIRANDQHLTAAAIHNLDEAVKFDMGAYTSSKDDTKVPVILRISKTQLYVSAQDEDQPVLLKEMPEINKTITGSETNFLFFWETHGTKNYFISVAHPNLFIATKHDNWVCLAKGLPSITDFQILENQA

Approximately 18.1 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.