IL-1 beta 蛋白, Human (C-His)

IL-1 beta protein is a potent proinflammatory cytokine that plays multiple roles in immune responses. It induces inflammatory events including prostaglandin synthesis, neutrophil activation, and cytokine production. IL-1 beta Protein, Human (C-His) is the recombinant human-derived IL-1 beta protein, expressed by E. coli , with C-6*His labeled tag. The total length of IL-1 beta Protein, Human (C-His) is 153 a.a., with molecular weight of ~19.0 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，因其在协调免疫反应中的多种作用而受到认可。IL-1β最初被认为是一种主要的内源性热原，可诱导一系列炎症事件，包括前列腺素合成、中性粒细胞流入和激活、T细胞和B细胞激活、细胞因子产生以及成纤维细胞增殖和胶原蛋白产生。它在免疫细胞分化中发挥着关键作用，促进 T 细胞的 Th17 分化，并与 IL-12 协同诱导辅助性 T 1 (Th1) 细胞合成 IFNG。此外，IL-1β 通过诱导 VEGF 产生，与 TNF 和 IL-6 协同作用，促进血管生成。值得注意的是，它在细胞焦亡下游炎症转导中发挥着关键作用，通过gasdermin-D (GSDMD) 孔特异性释放到细胞外环境中。在微生物感染的情况下，IL-1β充当皮肤中化脓性链球菌感染的传感器，被化脓性链球菌SpeB蛋白酶裂解和激活，导致炎症反应，从而在侵袭性皮肤感染期间抑制细菌生长。然而，SpeB 对 IL-1β 的裂解具有双重作用，通过破坏微生物群介导的定植抵抗来促进鼻咽链球菌感染。

Measured in a cell proliferation assay using CTLL-2 cells. The ED₅₀ for this effect is 1.67 pg/mL, corresponding to a specific activity is 5.99×10⁸ units/mg.

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  Measured in a cell proliferation assay using CTLL-2 cells.The ED₅₀ for this effect is 1.67 pg/mL, corresponding to a specific activity is 5.99×10⁸ units/mg.

Human

E. coli

C-6*His

P01584 (A117-S269)

3553

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Approximately 19.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.