IL-1 beta 蛋白, Human

IL-1 beta is a potent proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta plays a key role in inflammation and immunologic reactions^[1][2]. IL-1 beta is produced as inactive pro-IL-1β (encoded by pro-Il-1b) in response to inflammatory stimuli. IL-1 beta Protein, Human consists of 153 amino acids (A117-S269) and is expressed in E. coli.

白细胞介素-1β (IL-1β) 是一种促炎细胞因子，由多种细胞类型产生和分泌，尽管绝大多数研究都集中在先天免疫系统细胞（例如单核细胞和单核细胞）内的产生。巨噬细胞^[1][2]。
IL-1β 产生为无活性的 pro-IL-1β（由 pro-IL-1b 编码），以响应炎症刺激，包括微生物产物和内源性危险相关分子。IL-1β 基因表达和前 IL-1β 合成在模式识别受体 (PRR) 激活后发生。炎症刺激还会驱动含有 CARD 和 PYHIN 结构域的 PRR 的激活，这些 PRR 会募集 ASC 和 caspase-1 (Casp-1) 组装成多蛋白复合物炎症小体。Pro-Casp-1（由 pro-Casp-1 编码）由炎症小体激活，将 pro-IL-1β 裂解为具有生物活性的 IL-1β。IL-1β 通过 I 型 IL-1 受体 (IL-1R1) 以自分泌/旁分泌方式发挥作用^[1][2][3].
IL-1β可以调节炎症反应，参与多种细胞活动，包括细胞增殖、分化和凋亡。IL-1β 还对雌性^[1][2][3]的生殖起着重要的调节作用。

IL-1β (1-15625 pg/mL; 12 hours) dose-dependently increases production of the NF-κB-dependent inflammatory cytokine TNF-α, and a significant effect of IL-1β is observed at concentrations as low as 5 pg/mL in immortalized murine RAW264.7 macrophages^[1].
IL-1β (1, 10 and 100 ng/mL; 24 hours) results in a dose-dependent increase of cell proliferation in pig heart cells. IL-1β increases the gene expression of caspase-3, and increases the enzymatic activities of MMP-2 and MMP-9^[2].

IL-1β (8 ng; i.p.; daily; for 3 days) rescues Casp1^−/− mice, restores survival and reduces lung bacterial load (i.e. days 0, 1, and 2 post-infection) in Chlamydia pneumoniae infected Casp1^−/− mice. Yet, mice treated later (days 2, 3 and 4 post-infection) shows significantly increased bacterial counts relative to early-treated mice^[3].

1.The ED₅₀ is <10 pg/mL as measured by mouse D10S cells, corresponding to a specific activity of 1.0 × 10⁸ IU/mg.
2.Measured by its ability to induce NF-kB signaling in 293-IL1 Res cells. The ED₅₀ for this effect is 20-100 pg/mL.
3. Measured in a proliferation assay using CTLL-2 mouse cells. The ED₅₀  this effect is ≤12.41 pg/mL, corresponding to a specific activity is ≥8.058×10⁷ units/mg.

• 
  Measured in a proliferation assay using CTLL-2 mouse cells. The ED₅₀  this effect is 12.41 pg/mL, corresponding to a specific activity is 8.058×10⁷ units/mg.

Human

E. coli

Tag Free

P01584 (A117-S269)

3553  [NCBI]

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Approximately 18.94 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM Tris-HCl, 150 mM NaCl, pH 7.5 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jan Petrasek, et al. IL-1 receptor antagonist ameliorates inflammasome-dependent alcoholic steatohepatitis in mice. J Clin Invest. 2012 Oct;122(10):3476-89.  [Content Brief]

  [2]. Karina Zitta, et al. Interleukin-1beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells. Biochem Biophys Res Commun. 2010 Sep 3;399(4):542-7.  [Content Brief]

  [3]. Kenichi Shimada, et al. Caspase-1 dependent IL-1β secretion is critical for host defense in a mouse model of Chlamydia pneumoniae lung infection. PLoS One. 2011;6(6):e21477.  [Content Brief]