IL-10 蛋白, Pig

IL-10 Protein, a key immune regulator, mitigates inflammation by binding to its receptor (IL10RA/IL10RB), activating JAK1 and STAT2, leading to STAT3-mediated anti-inflammatory gene expression. IL-10 targets APCs, reducing pro-inflammatory cytokines and hindering antigen presentation. It controls macrophage inflammation through metabolic reprogramming. Existing as a homodimer, IL-10 interacts with IL10RA/IL10RB. IL-10 Protein, Pig is the recombinant pig-derived IL-10 protein, expressed by E. coli , with tag free. The total length of IL-10 Protein, Pig is 157 a.a., with molecular weight of ~18.0 kDa.

IL-10是一种主要的免疫调节细胞因子，通过发挥深远的抗炎功能，有效限制炎症引起的过度组织破坏，在调节免疫系统中发挥着关键作用。从机制上讲，IL-10 与其异四聚体受体（由 IL10RA 和 IL10RB 组成）结合，启动 JAK1 和 STAT2 介导的 STAT3 磷酸化。随后，磷酸化的 STAT3 易位至细胞核，驱动抗炎介质的表达。IL-10 特异性靶向抗原呈递细胞 (APC)，例如巨噬细胞和单核细胞，抑制其释放促炎细胞因子，包括 GM-CSF、G-CSF、IL-1 α、IL-1 β、IL-6 、IL-8 和 TNF-α。此外，IL-10 通过减少 MHC II 类和共刺激分子的表达来干扰抗原呈递，从而阻碍它们诱导 T 细胞激活的能力。此外，IL-10 通过重新编程重要的代谢途径（包括 mTOR 信号传导）来维持对巨噬细胞炎症反应的控制。在结构上，IL-10 形成同二聚体，并在其调节功能中与 IL10RA 和 IL10RB 结合。

1.Measured by its binding ability in a functional ELISA. Immobilized IL-10 Protein, Pig at 2 μg/mL (100 μl/well) can bind Rhesus IL10RA-hFc and the EC₅₀ is 25-100 ng/mL.
2.Measured in a cell proliferation assay using MC/9-2 mouse mast cells and the ED₅₀ is typically 1-5 ng/mL.

Pig

E. coli

Tag Free

Q29055 (S19-N175)

397106  [NCBI]

Approximately 18.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.