1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-10
  5. IL-10 蛋白, Rat

IL-10 蛋白, Rat

目录号: HY-P7098
COA 产品使用指南

IL-10 蛋白, Rat是由多种哺乳动物细胞类型产生的免疫抑制细胞因子,包括巨噬细胞,单核细胞,B细胞,T细胞和角质形成细胞。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥520 In-stock
10 μg ¥1350 In-stock
50 μg ¥3500 In-stock
100 μg ¥5600 询价
> 100 μg   询价  

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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-10 Protein, Rat is an immunosuppressive cytokine produced by a variety of mammalian cell types including macophages, monocytes, B-cells, T-cells and keratinocytes.

研究背景

Interleukin-10 是一种抗炎 Th1 免疫抑制细胞因子,其活性形式是非共价同二聚体,在结构和生物活性方面均表现出物种特异性。IL-10 的大鼠同源物与人 IL-10 在氨基酸序列水平上具有 73% 的同一性,并且除了人 IL-10 中存在的两个二硫键外,还具有第五个未配对的半胱氨酸 (cys-149) )。通过细菌表达制备大鼠 IL-10,然后在谷胱甘肽氧化还原系统中溶解和重折叠,产生其中 cys-149 几乎完全氧化的分子,以二硫化物二聚体或与谷胱甘肽混合的二硫化物形式存在,并且具有低于分子天然 (cys-149-SH) 形式活性的 1%。对大鼠 IL-10 进行定点诱变,用酪氨酸取代 cys-149,产生一种分子,该分子在溶解和重折叠时很容易采用活性构象,并且可以从细菌表达系统中以良好的产率回收。大鼠 IL-10tyr149 和商业大鼠 IL-10 制剂的生物活性比较证实,天然序列大鼠 IL-10 的活性降低或不存在。因此,建议生物合成类似物ratIL-10tyr149是研究IL-10在大鼠体内生物学作用的更有用的分子[1]

生物活性

Measured in a cell proliferation assay using HepG2. The EDED50 for this effect is 2.982 ng/mL. corresponding to a specific activity is 3.35×105 units/mg.

种属

Rat

表达系统

E. coli

标签

Tag Free

蛋白编号

P29456 (S19-N178)

基因 ID
蛋白结构
N-term
IL-10 (S19-N178)
Accession # P29456
C-term
同用名
rRtIL-10; CSIF
氨基酸序列

SKGHSIRGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNILLTDSLLQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVEQVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN

分子量

Approximately 18.7 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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