IL-10R alpha 蛋白, Rhesus Macaque (HEK293, His)

IL-10R alpha protein is an IL10 cytokine surface receptor that is involved in IL10-mediated inflammation and immune regulation and inhibits the synthesis of pro-inflammatory cytokines. IL-10R alpha Protein, Rhesus Macaque (HEK293, His) is expressed in HEK 293 cells and consists of 590 amino acids (M1-S590) with a His tag at the C-terminus^[1].

IL-10Rα是II型细胞因子受体的配体结合亚基，由2个α和2个β亚基组成，主要在造血细胞中表达，例如B细胞、T细胞、NK细胞、单核细胞和巨噬细胞，但不在非造血细胞中表达。-造血细胞，如成纤维细胞或内皮细胞^[1]。
IL-10R α 与配体结合并导致 β 亚基发生构象变化，从而导致 β 亚基也发生构象变化结合 IL-10，形成异四聚体，从而激活 JAK1 和 TYK2 激酶的信号复合物。在这种情况下，JAK1 与 α 亚基结合，TYK2 与 β 亚基结合，磷酸化 IL10R α 细胞内结构域中的特定酪氨酸残基。这进一步导致转录因子 STAT3 的磷酸化和激活，从而使 STAT3 单体二聚化进入细胞核并诱导靶基因的转录表达^[1]。
IL-10R α 参与抑制炎症反应和 Th 1 细胞介导的免疫反应，还调节中性粒细胞功能反应。此外，IL10R α 介导的 STAT3 激活也会抑制饥饿诱导的自噬^[2]。

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-10 is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Rhesus Macaque IL-10R alpha. The ED₅₀ for this effect is 17.16 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human IL-10 is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Rhesus Macaque IL-10R alpha. The ED₅₀ for this effect is 17.16 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_001092736 (F29-N245)

704383  [NCBI]

FFSPGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGTGRWNSISNCSQALSYDLTAVTLDLYRSNGYWARVRAVDGSRHSNWTVTNTRFSLDEVTLTVGSVKLEIHNGFILGKIQPPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVTSRTNKGMWSKEECVSLTRQYFTVTN

Approximately 35-50 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Dror S Shouval, et al. Interleukin 10 receptor signaling: master regulator of intestinal mucosal homeostasis in mice and humans. Adv Immunol. 2014;122:177-210.  [Content Brief]

  [2]. J Shi, et al. IL10 inhibits starvation-induced autophagy in hypertrophic scar fibroblasts via cross talk between the IL10-IL10R-STAT3 and IL10-AKT-mTOR pathways. Cell Death Dis. 2016 Mar 10;7(3):e2133.  [Content Brief]