IL-11 蛋白, Mouse

IL-11 protein is a multifunctional cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation to increase platelet production. It also contributes to liver cell proliferation in response to liver injury. IL-11 Protein, Mouse is the recombinant mouse-derived IL-11 protein, expressed by E. coli , with tag free.

IL-11 蛋白是一种具有多方面作用的多功能细胞因子，刺激造血干细胞和巨核细胞祖细胞的增殖，同时诱导巨核细胞成熟，从而提高血小板产量。此外，IL-11 有助于肝细胞增殖以应对肝损伤。IL-11 与其受体（由 IL6ST 和 IL11RA1 或 IL11RA2 形成）结合后，会激活信号级联，促进细胞增殖，这一过程与多种癌症有关。该信号传导触发细胞内蛋白激酶的激活和 STAT3 的磷酸化。值得注意的是，与膜结合的 IL11RA 和 IL6ST 的相互作用导致“经典信号传导”，而可溶性 IL11RA 与 IL6ST 的结合则刺激“反式信号传导”。IL-11 通过与 IL11RA1 或 IL11RA2 相互作用进一步形成多聚体信号复合物，突显其在协调多种细胞反应中的关键作用。

Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is 5.863 ng/mL, corresponding to a specific activity is 1.705×10⁵ U/mg.

Mouse

E. coli

Tag Free

P47873/NP_032376.1 (P22-L199)

16156  [NCBI]

PGPPAGSPRVSSDPRADLDSAVLLTRSLLADTRQLAAQMRDKFPADGDHSLDSLPTLAMSAGTLGSLQLPGVLTRLRVDLMSYLRHVQWLRRAGGPSLKTLEPELGALQARLERLLRRLQLLMSRLALPQAAPDQPVIPLGPPASAWGSIRAAHAILGGLHLTLDWAVRGLLLLKTRL

Approximately 20 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.