IL-12R beta 2 蛋白, Mouse (HEK293, Fc)

IL-12R beta 2 protein is an IL-12 cytokine surface receptor that activates the Jak-Stat signaling cascade pathway and is involved in IL-12-mediated immune regulation. IL-12R beta 2 Protein, Mouse (HEK293, Fc) is expressed by HEK293 cells with a hFc tag at the C-terminus^[1].

IL-12受体是与IL-12结合的I型细胞因子受体。IL-12 受体主要在 NK 和 T 细胞上表达，由 β1 和 β2 亚基组成，是 gp130 细胞因子受体超家族的成员。IL-12Rβ2（缩写为IL-12R2或IL-12Rβ2）是IL-12受体的一个亚基，IL-12Rβ2是其人类基因名称。IL-12Rβ2蛋白和IL-12Rβ1蛋白的共表达可以导致高亲和力IL-12结合位点的形成以及IL-12依赖性信号传导的重建。IL-12 与 IL-12 受体结合后，直接与 IL-12Rβ1 相互作用的细胞质蛋白 TYK2 和与 IL-12Rβ2 相互作用的 JAK2 被酪氨酸磷酸化。随后的酪氨酸磷酸化和与 IL-12Rβ2 结合的 STAT4 的激活需要磷酸化的 TYK2 和 JAK2。STAT4 是一种转录因子，随后同二聚化、易位至细胞核并与其靶 DNA 结合，激活 IFN-γ 和其他靶基因的转录^[1]。
IL- 的表达12Rβ2链被认为是控制T细胞发育和功能的关键因素。它在 Th1 细胞分化中发挥重要作用，缺失会导致 Th1 分化失败和 Th1 效应分子产生功能失调。人们发现该基因的上调与许多传染病有关，例如克罗恩病和麻风病，这些疾病被认为有助于炎症反应和宿主防御^[2]。

The number of Treg cells in the resting spleen of IL-12Rβ2^-/- mice is comparable to that of strain-matched wild-type (WT) mice. Among them, IL-12Rβ2^-/- splenocytes are completely resistant to IL-12-mediated inhibition and lacked detectable IL-12 receptor mRNA in reg cells, suggesting that IL-12 signaling can indirectly inhibit Treg amplification^[3].
IL-12Rβ2 is expressed primarily in NK cells, and mice lacking the IL-12Rβ2 subunit exhibit transient weight loss, the severity of which correlates with serum cytokine levels of IFN-γ. And, serum levels of IL-1β and IL-23 can be detected in IL-12Rβ^-/-.Rag1^-/-mice on C57BL/6 background, even in the absence of anti-CD40 treatment. IL-12Rβ2^-/- .Rag1^-/- mice exhibits elevated mRNA levels of most pro-inflammatory cytokines^[4].

Mouse

HEK293

C-hFc

P97378 (N24-N637)

16162  [NCBI]

MAQTVRECSLALLFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSAANISCSLNPKQGCSHYPSSNELILLKFVNDVLVENLHGKKVHDHTGHSSTFQVTNLSLGMTLFVCKLNCSNSQKKPPVPVCGVEISVGVAPEPPQNISCVQEGENGTVACSWNSGKVTYLKTNYTLQLSGPNNLTCQKQCFSDNRQNCNRLDLGINLSPDLAESRFIVRVTAINDLGNSSSLPHTFTFLDIVIPLPPWDIRINFLNASGSRGTLQWEDEGQVVLNQLRYQPLNSTSWNMVNATNAKGKYDLRDLRPFTEYEFQISSKLHLSGGSWSNWSESLRTRTPEEEPVGILDIWYMKQDIDYDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTSWTRVIPRTGAWTASVSAANSKGASAPTHINIVDLCGTGLLAPHQVSAKSENMDNILVTWQPPKKADSAVREYIVEWRALQPGSITKFPPHWLRIPPDNMSALISENIKPYICYEIRVHALSESQGGCSSIRGDSKHKAPVSGPHITAITEKKERLFISWTHIPFPEQRGCILHYRIYWKERDSTAQPELCEIQYRRSQNSHPISSLQPRVTYVLWMTAVTAAGESPQGNEREFCPQGKAN

125-160 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Esther van de Vosse, et al. IL-12Rβ1 deficiency: mutation update and description of the IL12RB1 variation database. Hum Mutat. 2013 Oct;34(10):1329-39.  [Content Brief]

  [2]. Ryuta Nishikomori, et al. Activated STAT4 has an essential role in Th1 differentiation and proliferation that is independent of its role in the maintenance of IL-12R beta 2 chain expression and signaling. J Immunol. 2002 Oct 15;169(8):4388-98.  [Content Brief]

  [3]. Xuefang Cao, et al. Interleukin 12 stimulates IFN-gamma-mediated inhibition of tumor-induced regulatory T-cell proliferation and enhances tumor clearance. Cancer Res. 2009 Nov 15;69(22):8700-9.  [Content Brief]

  [4]. Gaëlle Chognard, et al. The dichotomous pattern of IL-12r and IL-23R expression elucidates the role of IL-12 and IL-23 in inflammation. PLoS One. 2014 Feb 21;9(2):e89092.  [Content Brief]