IL-13 蛋白, Human (CHO)

IL-13 Protein, Human (CHO) is a CHO cell derived cytokine which affects the morphology, growth, and surface antigen expression and phenotype of monocytes and elicits B cell proliferation.

Interleukin-13 是一种细胞因子，由活化的 T 淋巴细胞分泌，主要影响单核细胞、巨噬细胞和 B 细胞。圆二色光谱证实 IL-13 属于细胞因子的 α 螺旋家族。Interleukin-13 影响单核细胞的形态、生长、表面抗原表达和表型，并引发 B 细胞增殖、Ig 类别转换为 IgE 合成以及 IgG4 和 IgM 的产生增强。在人类巨噬细胞和单核细胞中，hIL-13 已被证明可以抑制 HIV 复制。人 IL-13 还可抑制 LPS 暴露诱导的促炎细胞因子，表明作为抗炎剂具有潜在的治疗应用^[1]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is ≤24.04 ng/mL, corresponding to a specific activity is ≥4.20×10⁴ U/mg.

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  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 6.131 ng/ml, corresponding to a specific activity is 1.63×10⁵ U/mg.

Human

CHO

Tag Free

P35225 (S33-N146)

3596  [NCBI]

SPGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGRFN

10-25 kDa, under reducing conditions & 25-45 kDa, under non-reducing conditions

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Cannon-Carlson S, et al. Expression, purification, and characterization of recombinant human interleukin-13 from NS-O cells. Protein Expr Purif. 1998 Mar;12(2):239-48.  [Content Brief]