IL-17F 蛋白, Human

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer^[1][2]. IL-17F Protein, Human is a recombinant human IL-17F protein and is expressed in E. coli. It consists of 133 amino acids (R31-Q163).

白细胞介素-17F (IL-17F) 属于 IL-17 细胞因子家族。IL-17F 在活化的 CD4 T 细胞、活化的单核细胞、嗜碱性粒细胞和肥大细胞中表达。IL-17F 可由分化的 TH17 细胞、固有层 T 细胞、记忆 CD4⁺ T 细胞、γδ T 细胞和 NKT 细胞^[1]产生。
人IL-17F与小鼠的氨基酸序列同一性为55.90%，与大鼠的氨基酸序列同一性为57.14%。
IL-17F是一种炎症细胞因子，可诱导多种促炎细胞因子和趋化因子，包括TGF-β、IL-2、ICAM1、GM-CSF、CCL2、CCL7、TSLP、MMP13、IL-6和CXCL1。IL-17F还与IL-22一起诱导抗菌肽，包括hBD-2、S100A7、S100A8和S100A9，并且可以与人嗜酸性粒细胞中的IL-23协同作用，促进IL-1β和IL-6的产生。IL-17F 是同型二聚体细胞因子。IL-17F 与 IL-17A 最相似（50% 同源性），并且可以作为 IL-17AF 异二聚体产生。IL-17A、IL-17F和IL-17A/F使用相同的受体复合物：IL-17RA和IL-17RC异二聚体。它们触发性质相似的信号通路，并且 IL-17F 表现出最低的生物活性。IL-17F 对 IL-17RA 亚基的亲和力比 IL-17A 低约 100–1000 倍，并且不与 IL-17A 竞争与 IL-17RA 的结合^[1][2]。
IL-17F 在结肠癌发展中发挥保护作用，可用于自身免疫性疾病、感染和癌症的研究^[1][3][4]。

IL-17F (50 ng/mL; 16-24 h) induces GRO-α, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells^[5].
IL-17F binds weakly to IL-17RA.Fc with an EC₅₀ > 2000 ng/mL, whereas IL-17A binds IL-17RA.Fc with a value of 19 ng/mL, the IL-17F/IL-17A heterodimer has an EC50 of 329 ng/mL^[5].
IL-17F (0-150 ng/mL; 0-72 h) increases the cell viability of PC-3 and DU145^[6].
IL-17F (100 ng/mL; 24 h) stimulates the malignant phenotypes of PCa cells and activates PI3K/AKT signalling pathway in PCa cells^[6].

The ED₅₀ is <40 ng/mL as measured by murine NIH/3T3 cells, corresponding to a specific activity of >5.0 × 10⁴ units/mg.

Human

E. coli

Tag Free

Q96PD4 (R31-Q163)

112744  [NCBI]

RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ

15-32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, pH 7.2, with trehalose or 20 mM PB, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Chang SH, et al. IL-17F: regulation, signaling and function in inflammation. Cytokine. 2009 Apr;46(1):7-11.  [Content Brief]

  [2]. Oda N, et al. Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response. Am J Respir Crit Care Med. 2005 Jan 1;171(1):12-8.  [Content Brief]

  [3]. McGeachy MJ, et al. The IL-17 Family of Cytokines in Health and Disease. Immunity. 2019 Apr 16;50(4):892-906.  [Content Brief]

  [4]. Tong Z, et al. A protective role by interleukin-17F in colon tumorigenesis. PLoS One. 2012;7(4):e34959.  [Content Brief]

  [5]. Ferreira N, et al. IL-17A and IL-17F orchestrate macrophages to promote lung cancer. Cell Oncol (Dordr). 2020 Aug;43(4):643-654.  [Content Brief]

  [6]. Tong Z, et al. A protective role by interleukin-17F in colon tumorigenesis. PLoS One. 2012;7(4):e34959.  [Content Brief]

  [7]. Wright JF, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008 Aug 15;181(4):2799-805.  [Content Brief]

  [8]. Zhu X, et al. IL-17F facilitates prostate cancer cell malignant phenotypes via activation of the PI3K/AKT signalling pathway. Andrologia. 2020 Nov;52(10):e13750.  [Content Brief]