IL-18BP 蛋白, Human (CHO)

IL-18BP Protein, Human (CHO) Binding Protein (CHO-expressed) is a modulator of the Th1 cytokine response.

IL18BP（Interleukin-18 结合蛋白）是 1 型 T 淋巴细胞辅助细胞 (Th1) 反应发展的早期信号^[1]。IL18BP与IL1家族的可溶性细胞因子受体有许多共同特征，即该蛋白对IL18表现出特异性，属于免疫球蛋白样受体类别，并且具有与II型IL1受体相同的有限氨基酸序列。IL18BP 抑制 IFNγ 的产生，导致 1 型辅助 T 免疫反应减少。两种人 IL18BP 亚型对 IL18 表现出最大的亲和力，具有快速的结合速率、缓慢的解离速率和 399 pM 的解离常数 (kDa)。其他两种具有不完整免疫球蛋白结构域的亚型无法中和 IL18^[2]。

The ED₅₀ is <30 ng/mL as measured by KG-1 cells.

Human

CHO

Tag Free

O95998-2 (T31-G194)

10068  [NCBI]

TPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQQG

42-44 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Novick D, et al. Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response. Immunity. 1999 Jan;10(1):127-36.  [Content Brief]

  [2]. C. Dinarello, et al. Targeting interleukin 18 with interleukin 18 binding protein. Ann Rheum Dis. 2000 Nov; 59(Suppl 1): i17–i20.