IL-19 蛋白, Mouse

IL-19 Protein, Mouse is a member of the Interleukin-10 family. The biological functions and clinical implications of Interleukin-19 is still undefined.

IL-19 与 IL-10 具有 21% 的氨基酸同一性。IL-19的外显子/内含子结构与人IL-10基因的外显子/内含子结构相似，在IL-19 cDNA的编码区内包含五个外显子和四个内含子。IL-19 不结合经典 IL-10 受体复合物或通过其发出信号^[1]。IL-19 通过由 IL-20Ra 和 IL20Rb 组成的受体复合物发挥作用，IL-20 和 IL-24 也利用该受体复合物。IL19 已被证明可以增强 T 细胞中 Th2 细胞因子的产生，并且在哮喘患者中升高。此外，它还诱导单核细胞中 IL-6、IL-8 和 IL-10 的表达。此外，它还与一系列疾病和紊乱有关，包括衰老、I 型糖尿病、内毒素性休克、牙周病、血管疾病和类风湿性关节炎^[2]。

The ED₅₀ is <50 ng/mL as measured by MCF-7 cells, corresponding to a specific activity of >2.0 × 10⁴ units/mg.

• 
  Measured by a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is 24.92 ng/mL,corresponding to a specific activity is 4.013×10⁴ units/mg.

Mouse

E. coli

Tag Free

Q8CJ70 (L25-A176)

329244  [NCBI]

LRRCLISVDMRLIEKSFHEIKRAMQTKDTFKNVTILSLENLRSIKPGDVCCMTNNLLTFYRDRVFQDHQERSLEVLRRISSIANSFLCVQKSLERCQVHRQCNCSQEATNATRIIHDNYNQLEVSSAALKSLGELNILLAWIDRNHLETPAA

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 50 mM acetic acid or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

Reconstitute the lyophilized powder up to 50 μg/mL in 50 mM acetic acid. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. IL-19 shares 21% amino acid identity with IL-10. The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA. IL-19 does not bind or signal through the canonical IL-10 receptor complex^[1]. IL-19 functions through a receptor complex composed of IL-20Ra and IL20Rb that is also utilized by IL-20 and IL-24. IL19 has been shown to enhance the production of Th2 cytokines in Tcells and to be elevated in asthma patients. Furthermore, it induces IL-6, IL-8 and IL-10 expression in monocytes. Additionally, it has been implicated in a range of diseases and disorders, including aging, Type-I diabetes, endotoxic shock, periodontal disease, vascular disease and rheumatoid arthritis^[2].  [Content Brief]

  [2]. Gallagher G, et al. Interleukin-19: multiple roles in immune regulation and disease. Cytokine Growth Factor Rev. 2010 Oct;21(5):345-52.  [Content Brief]